Western Blot (WB)
(Western Blot: Sample: Mouse Liver lysate; Primary Ab: 2ug/mL Rabbit Anti-Mouse DGKa Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Mouse Diacylglycerol Kinase Alpha Polyclonal Antibody | anti-DGKa antibody
Polyclonal Antibody to Diacylglycerol Kinase Alpha (DGKa)
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Avoid repeated freeze-thaw cycles.
Store at 4°C for frequent use.
Aliquot and store at -20°C for 24 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Mouse Liver lysate; Primary Ab: 2ug/mL Rabbit Anti-Mouse DGKa Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Mouse Liver lysate; Primary Ab: 2ug/mL Rabbit Anti-Mouse DGKa Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
NCBI and Uniprot Product Information
Uniprot Description
DGKA: Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity. Monomer. Lymphocytes and oligodendroglial cells. Stimulated by calcium and phosphatidylserine. Phosphorylated by protein kinase C. Belongs to the eukaryotic diacylglycerol kinase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.7.1.107; Kinase, lipid; Lipid Metabolism - glycerolipid; Lipid Metabolism - glycerophospholipid; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 10 D3|10 77.14 cM
Cellular Component: cytosol; membrane; plasma membrane
Molecular Function: ATP binding; calcium ion binding; diacylglycerol kinase activity; kinase activity; metal ion binding; NAD+ kinase activity; nucleotide binding; phospholipid binding; transferase activity
Biological Process: diacylglycerol metabolic process; glycerolipid metabolic process; lipid phosphorylation; metabolic process; phosphatidic acid biosynthetic process; phosphorylation; protein kinase C-activating G-protein coupled receptor signaling pathway
Research Articles on DGKa
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Product Notes
The DGKa dgka (Catalog #AAA2027285) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Diacylglycerol Kinase Alpha (DGKa) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Diacylglycerol Kinase Alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the DGKa dgka for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Diacylglycerol Kinase Alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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