DDIT4 recombinant protein

DDIT4, 1-232aa, Human, His tag, E Coli

Cat. Num. AAA203808

• Gene Names: DDIT4; Dig2; REDD1; REDD-1
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: DDIT4; 1-232aa; Human; His tag; E Coli; DNA damage-inducible transcript 4 protein; Dig2; FLJ20500; REDD1; RP11-442H21.1; RTP801; DDIT4 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 1mM EDTA, 30% glycerol
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MPSLWDRFSS SSTSSSPSSL PRTPTPDRPP RSAWGSATRE EGFDRSTSLE SSDCESLDSS NSGFGPEEDT AYLDGVSLPD FELLSDPEDE HLCANLMQLL QESLAQARLG SRRPARLLMP SQLVSQVGKE LLRLAYSEPC GLRGALLDVC VEQGKSCHSV GQLALDPSLV PTFQLTLVLR LDSRLWPKIQ GLFSSANSPF LPGFSQSLTL STGFRVIKKK LYSSEQLLIE EC
• Sequence Length: 232

• Applicable Applications for DDIT4 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for DDIT4 recombinant protein

DDIT4, also known as Dig2 or REDD1, is thought to have function in the regulation of reactive oxygen species. In response to stress due to DNA damage and glucocorticoid treatment, DDIT4 is upregulated at the transcriptional level. DDIT4 negatively regulates the mammalian target of Rapamycin, a serine/threonine kinase often referred to as mTOR. It is crucial in the coupling of extra- and intracellular cues to mTOR regulation. Recombinant human DDIT4 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for DDIT4 recombinant protein

Cancer

References

Yoshida T., et al. (2010) Nat Med. 16 (7):767-73.; Regazzetti C., et al. (2010) J Biol Chem. 285 (8):5157-64.

NCBI and Uniprot Product Information

• NCBI GI #: 9506687
• NCBI GeneID: 54541
• NCBI Accession #: NP_061931
• NCBI GenBank Nucleotide #: NM_019058.3
• UniProt Accession #: Q9NX09; Q9H0S3
• Molecular Weight: 27.5 kDa (252aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
• NCBI Official Full Name: DNA damage-inducible transcript 4 protein
• NCBI Official Synonym Full Names: DNA damage inducible transcript 4
• NCBI Official Symbol: DDIT4
• NCBI Official Synonym Symbols: Dig2; REDD1; REDD-1
• NCBI Protein Information: DNA damage-inducible transcript 4 protein
• UniProt Protein Name: DNA damage-inducible transcript 4 protein
• Protein Family: DNA damage-inducible transcript 4 protein
• UniProt Gene Name: DDIT4
• UniProt Synonym Gene Names: REDD1; RTP801; REDD-1

Uniprot Description

Regulates cell growth, proliferation and survival via inhibition of the activity of the mammalian target of rapamycin complex 1 (mTORC1). Inhibition of mTORC1 is mediated by a pathway that involves DDIT4/REDD1, AKT1, the TSC1-TSC2 complex and the GTPase RHEB. Plays an important role in responses to cellular energy levels and cellular stress, including responses to hypoxia and DNA damage. Regulates p53/TP53-mediated apoptosis in response to DNA damage via its effect on mTORC1 activity. Its role in the response to hypoxia depends on the cell type; it mediates mTORC1 inhibition in fibroblasts and thymocytes, but not in hepatocytes (). Required for mTORC1-mediated defense against viral protein synthesis and virus replication (). Inhibits neuronal differentiation and neurite outgrowth mediated by NGF via its effect on mTORC1 activity. Required for normal neuron migration during embryonic brain development. Plays a role in neuronal cell death.