Rabbit DDIT4 Polyclonal Antibody | anti-DDIT4 antibody

DDIT4 Antibody

Cat. Num. AAA9608730

• Gene Names: DDIT4; Dig2; REDD1; REDD-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: DDIT4; Polyclonal Antibody; DDIT4 Antibody; DDIT4_HUMAN; Dig2; DNA damage inducible transcript 4; DNA damage inducible transcript 4 protein; DNA damage-inducible transcript 4 protein; FLJ20500; HIF 1 responsive protein RTP801; HIF 1 responsive RTP801; HIF-1 responsive protein RTP801; Protein regulated in development and DNA damage response 1; REDD-1; REDD1; RTP801; anti-DDIT4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: DDIT4 antibody detects endogenous levels of total DDIT4
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 232

• Applicable Applications for anti-DDIT4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-3000
• Immunogen: A synthesized peptide derived from human DDIT4
• Subcellular Location: Cytoplasm.
• Tissue Specificity: Broadly expressed, with lowest levels in brain, skeletal muscle and intestine. Up-regulated in substantia nigra neurons from Parkinson disease patients (at protein level).
• Predicted Cross Reactivity: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
• Similarity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (88%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of DDIT4 using Jurkat whole cell lysates)

Related Product Information for anti-DDIT4 antibody

Function: Regulates cell growth, proliferation and survival via inhibition of the activity of the mammalian target of rapamycin complex 1 (mTORC1). Inhibition of mTORC1 is mediated by a pathway that involves DDIT4/REDD1, AKT1, the TSC1-TSC2 complex and the GTPase RHEB. Plays an important role in responses to cellular energy levels and cellular stress, including responses to hypoxia and DNA damage. Regulates p53/TP53-mediated apoptosis in response to DNA damage via its effect on mTORC1 activity. Its role in the response to hypoxia depends on the cell type; it mediates mTORC1 inhibition in fibroblasts and thymocytes, but not in hepatocytes (By similarity). Required for mTORC1-mediated defense against viral protein synthesis and virus replication (By similarity). Inhibits neuronal differentiation and neurite outgrowth mediated by NGF via its effect on mTORC1 activity. Required for normal neuron migration during embryonic brain development. Plays a role in neuronal cell death.
Subunit Structure: Monomer. Interacts with BTRC. Identified in a complex with CUL4A, DDB1 and BTRC. Interacts with TXNIP; this inhibits the proteasomal degradation of DDIT4.
Post-translational Modifications: Phosphorylated by GSK3B; this promotes proteasomal degradation. Polyubiquitinated by a DCX (DDB1-CUL4A-RBX1) E3 ubiquitin-protein ligase complex with BTRC as substrate-recognition component, leading to its proteasomal degradation.
Similarity: Belongs to the DDIT4 family.

NCBI and Uniprot Product Information

• NCBI GI #: 9506687
• NCBI GeneID: 54541
• NCBI Accession #: NP_061931.1
• NCBI GenBank Nucleotide #: NM_019058.3
• UniProt Accession #: Q9NX09; Q9H0S3
• Molecular Weight: Observed: 25 kDa; Predicted: 26 kDa
• NCBI Official Full Name: DNA damage-inducible transcript 4 protein
• NCBI Official Synonym Full Names: DNA damage inducible transcript 4
• NCBI Official Symbol: DDIT4
• NCBI Official Synonym Symbols: Dig2; REDD1; REDD-1
• NCBI Protein Information: DNA damage-inducible transcript 4 protein
• UniProt Protein Name: DNA damage-inducible transcript 4 protein
• Protein Family: DNA damage-inducible transcript 4 protein
• UniProt Gene Name: DDIT4
• UniProt Synonym Gene Names: REDD1; RTP801; REDD-1

Uniprot Description

Regulates cell growth, proliferation and survival via inhibition of the activity of the mammalian target of rapamycin complex 1 (mTORC1). Inhibition of mTORC1 is mediated by a pathway that involves DDIT4/REDD1, AKT1, the TSC1-TSC2 complex and the GTPase RHEB. Plays an important role in responses to cellular energy levels and cellular stress, including responses to hypoxia and DNA damage. Regulates p53/TP53-mediated apoptosis in response to DNA damage via its effect on mTORC1 activity. Its role in the response to hypoxia depends on the cell type; it mediates mTORC1 inhibition in fibroblasts and thymocytes, but not in hepatocytes (). Required for mTORC1-mediated defense against viral protein synthesis and virus replication (). Inhibits neuronal differentiation and neurite outgrowth mediated by NGF via its effect on mTORC1 activity. Required for normal neuron migration during embryonic brain development. Plays a role in neuronal cell death.