Rabbit anti-Human, Mouse RTP801 Polyclonal Antibody | anti-DDIT4 antibody

RTP801 Antibody

Cat. Num. AAA150339

• Gene Names: DDIT4; Dig2; REDD1; REDD-1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: RTP801 Antibody is affinity chromatography purified via peptide column.
• Synonyms: RTP801; Polyclonal Antibody; RTP801 Antibody; Dig2; REDD1; REDD-1; DNA damage-inducible transcript 4 protein; HIF-1 responsive protein RTP801; DNA-damage-inducible transcript 4; anti-DDIT4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: RTP801 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 232

• Applicable Applications for anti-DDIT4 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: RTP801 antibody can be used for the detection of RTP801 by Western blot at 2 - 4 mug/mL.
• Conjugate: Unconjugated
• Immunogen: RTP801 antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human RTP801.
• Buffer: RTP801 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: RTP801 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of RTP801 in 293 cell lysate with RTP801 antibody at (A) 2 and (B) 4 μg/mL.)

Related Product Information for anti-DDIT4 antibody

RTP801 Antibody: RTP801 was initially identified as a gene induced by DNA damage, and later found to also be regulated by other cellular stresses such as hypoxia and glucocorticoid treatment. Recently, RTP801 has been shown to act as a mediator of tuberous sclerosis complex (TSC)-dependent regulation of the mammalian Target of Rapamycin (mTOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle. In response to energy stress, RTP801 inhibits mTOR function, resulting in dephosphorylation of downstream targets such as ribosomal protein S6 kinase 1 and 4EBP1 and decreasing cell growth. Disregulation of RTP801 may thus contribute to human tumorigenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 9506687
• NCBI GeneID: 54541
• NCBI Accession #: NP_061931
• NCBI GenBank Nucleotide #: NM_019058.2
• UniProt Accession #: Q9NX09; Q9H0S3
• Molecular Weight: 25,371 Da
• NCBI Official Full Name: DNA damage-inducible transcript 4 protein
• NCBI Official Synonym Full Names: DNA-damage-inducible transcript 4
• NCBI Official Symbol: DDIT4
• NCBI Official Synonym Symbols: Dig2; REDD1; REDD-1
• NCBI Protein Information: DNA damage-inducible transcript 4 protein; HIF-1 responsive protein RTP801; protein regulated in development and DNA damage response 1
• UniProt Protein Name: DNA damage-inducible transcript 4 protein
• Protein Family: DNA damage-inducible transcript 4 protein
• UniProt Gene Name: DDIT4
• UniProt Synonym Gene Names: REDD1; RTP801; REDD-1
• UniProt Entry Name: DDIT4_HUMAN

Uniprot Description

DDIT4: Inhibits cell growth by regulating the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. Promotes neuronal cell death. Up-regulated in fibroblasts upon ionizing radiation, via a TP53-dependent pathway. Up-regulated by TP63 in primary keratinocytes, and down-regulated during keratinocyte differentiation. Up-regulated upon DNA alkylation. Up-regulated by amyloid beta-peptide and retinoic acid. Up-regulated by hypoxia, via a PI3K and HIF1A-dependent but TP53/TP63-independent mechanism. Broadly expressed, with lowest levels in brain, skeletal muscle and intestine. Up-regulated in substantia nigra neurons from Parkinson disease patients. Belongs to the DDIT4 family.

Chromosomal Location of Human Ortholog: 10q22.1

Cellular Component: mitochondrion; cytoplasm; intracellular; cytosol

Biological Process: neuron differentiation; cell proliferation; negative regulation of TOR signaling pathway; protein complex disassembly; nerve growth factor receptor signaling pathway; neuron migration; response to hypoxia; negative regulation of peptidyl-serine phosphorylation; brain development; negative regulation of glycolysis; defense response to virus; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis