Mouse Cathepsin B ELISA Kit | CTSB elisa kit

Mouse Cathepsin B PicoKine ELISA Kit

Cat. Num. AAA175784

• Gene Names: Ctsb; CB
• Reactivity: Mouse
• Synonyms: Cathepsin B; Mouse Cathepsin B PicoKine ELISA Kit; cathepsin B; aAmyloid precursor protein secretase; APP secretase; APPS; CATB_HUMAN; Cathepsin B heavy chain; Cathepsin B1; CPSB; CTSB; Cysteine protease; CTSB elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse Ctsb
• Sequence Length: 339

• Samples: Cell culture supernatants, cell lysates, serum and plasma (heparin, edta)
• Detection Range: 156 pg/ml - 10,000 pg/ml
• Sensitivity: <5 pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CTSB elisa kit

Principle of the Assay: The Picokine™ Mouse Ctsb Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse Ctsb with a 96-well strip plate that is pre-coated with antibody specific for Ctsb. The detection antibody is a biotinylated antibody specific for Ctsb. The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Ctsb with immunogen: Expression system for standard: NS0; Immunogen sequence: H18-F339. The kit is analytically validated with ready-to-use reagents. To measure Mouse Ctsb, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The density of the yellow product is linearly proportional to Mouse Ctsb in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Ctsb in the sample.

Background/Introduction: Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene.1, 2 And this gene is mapped to chromosome 8p22.3 The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfidelinked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease.4 Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. The standard product used in this kit is recombinant mouse Cathepsin B with the molecular mass of 37KDa.

NCBI and Uniprot Product Information

• NCBI GI #: 115712
• NCBI GeneID: 13030
• UniProt Accession #: P10605; Q3UDW7
• Molecular Weight: 37,280 Da
• NCBI Official Full Name: Cathepsin B
• NCBI Official Synonym Full Names: cathepsin B
• NCBI Official Symbol: Ctsb
• NCBI Official Synonym Symbols: CB
• NCBI Protein Information: cathepsin B; cathepsin B1
• UniProt Protein Name: Cathepsin B
• Protein Family: Cathepsin
• UniProt Gene Name: Ctsb
• UniProt Entry Name: CATB_MOUSE

NCBI Description

This gene encodes a member of the peptidase C1 family and preproprotein that is proteolytically processed to generate multiple protein products. These products include the cathepsin B light and heavy chains, which can dimerize to generate the double chain form of the enzyme. This enzyme is a lysosomal cysteine protease with both endopeptidase and exopeptidase activity that may play a role in protein turnover. Homozygous knockout mice for this gene exhibit reduced pancreatic damage following induced pancreatitis and reduced hepatocyte apoptosis in a model of liver injury. Pseudogenes of this gene have been identified in the genome. [provided by RefSeq, Aug 2015]

Uniprot Description

CTSB: Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. Dimer of a heavy chain and a light chain cross-linked by a disulfide bond. Interacts with SRPX2. Belongs to the peptidase C1 family.

Protein type: Motility/polarity/chemotaxis; EC 3.4.22.1; Protease; Autophagy

Cellular Component: extracellular space; cell surface; intracellular membrane-bound organelle; mitochondrion; lysosome; extracellular region; caveola; perinuclear region of cytoplasm; apical plasma membrane; cytoplasm; nucleolus; sarcolemma; external side of plasma membrane

Molecular Function: peptidase activity; collagen binding; proteoglycan binding; protein self-association; hydrolase activity; cysteine-type endopeptidase activity; endopeptidase activity; protein complex binding; kininogen binding; peptide binding; cysteine-type peptidase activity

Biological Process: collagen catabolic process; proteolysis involved in cellular protein catabolic process; regulation of catalytic activity; decidualization; proteolysis