Western Blot (WB)
(Western blot analysis of Profilin1 expression in Jurkat cell lysate.)
Rabbit Profilin1 Polyclonal Antibody | anti-PFN1 antibody
Anti-Profilin1 Rabbit pAb
Gene Names
PFN1; ALS18
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
Affinity Chromatography
Synonyms
Profilin1; Polyclonal Antibody; Anti-Profilin1 Rabbit pAb; Actin binding protein; ALS18; Epididymis tissue protein Li 184a; PFN 1; Pfn; ProfilinI; anti-PFN1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Profilin1 Antibody detects endogenous levels of total Profilin1
Purity/Purification
Affinity Chromatography
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
140
Applicable Applications for anti-PFN1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-1:5000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50
FC/FACS: 1:200
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50
FC/FACS: 1:200
Immunogen
A synthesized peptide derived from human Profilin1
Subcellular Location
Cytoplasm; Cytoskeleton
Preparation and Storage
Store at 4 degree C for short term.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.
Western Blot (WB)
(Western blot analysis of Profilin1 expression in Jurkat cell lysate.)
Western Blot (WB)
(Western blot analysis of Profilin1 expression in Jurkat cell lysate.)
Related Product Information for anti-PFN1 antibody
Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15kDa
NCBI Official Full Name
profilin-1
NCBI Official Synonym Full Names
profilin 1
NCBI Official Symbol
PFN1
NCBI Official Synonym Symbols
ALS18
NCBI Protein Information
profilin-1
UniProt Protein Name
Profilin-1
UniProt Gene Name
PFN1
NCBI Description
This gene encodes a member of the profilin family of small actin-binding proteins. The encoded protein plays an important role in actin dynamics by regulating actin polymerization in response to extracellular signals. Deletion of this gene is associated with Miller-Dieker syndrome, and the encoded protein may also play a role in Huntington disease. Multiple pseudogenes of this gene are located on chromosome 1. [provided by RefSeq, Jul 2012]
Uniprot Description
Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR.
Research Articles on PFN1
Similar Products
Product Notes
The PFN1 pfn1 (Catalog #AAA4750882) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Profilin1 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Profilin1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:1000-1:5000 IHC: 1:50-1:200 ICC/IF: 1:50-1:200 IP: 1:50 FC/FACS: 1:200. Researchers should empirically determine the suitability of the PFN1 pfn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Profilin1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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