Human Cathepsin B ELISA Kit | CTSB elisa kit

Human Cathepsin B Quick ELISA Kit

Cat. Num. AAA1753925

• Reactivity: Human
• Synonyms: Cathepsin B; Human Cathepsin B Quick ELISA Kit; Cathepsin B|APP secretase; APPS; Cathepsin B1; CPSBamyloid precursor protein secretase; CTSB; cysteine protease; EC 3. 4. 22; EC 3. 4. 22. 1; cathepsin B; CTSB elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human CTSB

• Assay Type: Colorimetric sandwich
• Samples: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: <5pg/ml
• Capture/Detection Antibodies: Capture antibody- polyclonal antibody from goat|Detection antibody- polyclonal antibody from goat
• Sample Data: Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000; O.D.|0.107|0.243|0.348 |0.539|0.894|1.445|2.041|2.443
• Intra-Assay Precision: Sample|1|2|3|; n|16|16|16|; Mean(pg/ml)|242|1834|4362|; Standard deviation|16.7|126.55|322.79|; CV(%)|6.9%|6.9%|7.4%|
• Inter-Assay Precision: Sample|1|2|3|; n|24|24|24; Mean(pg/ml)|264|1932|4096; Standard deviation|20.86|142.97|372.74; CV(%)|7.9%|7.4%|9.1%
• Reproducibility: To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.; Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%); Sample 1: 242|251|245|277|254|15.99|6.3%; Sample 2: 1834|1763|1647|1547|1698|56.03|3.3%; Sample 3: 4362|4260|3862|4631|4279|282.4|6.6%; *number of samples for each test n=16.
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: R18-I339
• Anticoagulant: heparin or EDTA
• Benefits: The Quick ELISA kits, assay takes less than 1.5 hours. Detect Human Cathepsin B with <5pg/ml sensitivity. Format: 96-well plate with removable strips.; This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
• Principle: Human CTSB Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CTSB in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit contains recombinant Human CTSB with immunogen: Expression system for standard: NS0; Immunogen sequence: R18-I339.; ; To measure Human CTSB, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human CTSB in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CTSB in the sample.
• Assay Procedure Step 1: 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312.5pg/ml, 156.25pg/ml
• TMB Incubation Time: 15
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice.)

Testing Data

Related Product Information for CTSB elisa kit

Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene. 1, 2 And this gene is mapped to chromosome 8p22. 3 The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease. 4 Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. The standard product used in this kit is recombinant human Cathepsin B with the molecular mass of 37KDa.

Product Categories/Family for CTSB elisa kit

ELISA kits

NCBI and Uniprot Product Information

• NCBI GeneID: 1508
• UniProt Accession #: P07858
• UniProt Protein Name: Cathepsin B
• Protein Family: Cathepsin
• UniProt Gene Name: CTSB
• UniProt Synonym Gene Names: CPSB; APPS
• UniProt Entry Name: CATB_HUMAN

Uniprot Description

CTSB: Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. Dimer of a heavy chain and a light chain cross-linked by a disulfide bond. Interacts with SRPX2. Belongs to the peptidase C1 family.

Protein type: Autophagy; Motility/polarity/chemotaxis; EC 3.4.22.1; Protease

Chromosomal Location of Human Ortholog: 8p22

Cellular Component: extracellular space; mitochondrion; intracellular membrane-bound organelle; perinuclear region of cytoplasm; lysosome; nucleolus; extracellular region; melanosome; intracellular

Molecular Function: collagen binding; peptidase activity; proteoglycan binding; protein binding; cysteine-type endopeptidase activity; cysteine-type peptidase activity

Biological Process: regulation of apoptosis; extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; epithelial cell differentiation; proteolysis involved in cellular protein catabolic process; regulation of catalytic activity; toll-like receptor signaling pathway; innate immune response; decidualization; proteolysis