Rabbit anti-Human SKAP1 Polyclonal Antibody | anti-SKAP1 antibody

SKAP1, CT (SKAP1, SCAP1, SKAP55, Src kinase-associated phosphoprotein 1, Src family-associated phosphoprotein 1, Src kinase-associated phosphoprotein of 55kD) (AP)

Cat. Num. AAA6347912

• Gene Names: SKAP1; SCAP1; SKAP55; HEL-S-81p
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SKAP1; Polyclonal Antibody; CT (SKAP1; SCAP1; SKAP55; Src kinase-associated phosphoprotein 1; Src family-associated phosphoprotein 1; Src kinase-associated phosphoprotein of 55kD) (AP); Src kinase-associated phosphoprotein of 55kD; anti-SKAP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-SKAP1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SKAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic between 265-295 amino acids selected from the C-terminal region of human SKAP1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SKAP1 Antibody (C-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the SKAP1 antibody detected the SKAP1 protein (arrow).)

Related Product Information for anti-SKAP1 antibody

This gene encodes a T cell adaptor protein, a class of intracellular molecules with modular domains capable of recruiting additional proteins but that exhibit no intrinsic enzymatic activity. The encoded protein contains a unique N-terminal region followed by a PH domain and C-terminal SH3 domain. Along with the adhesion and degranulation-promoting adaptor protein, the encoded protein plays a critical role in inside-out signaling by coupling T-cell antigen receptor stimulation to the activation of integrins.

Product Categories/Family for anti-SKAP1 antibody

Antibodies; Protein Kinases

References

Goode, E.L., et al. Nat. Genet. 42(10):874-879(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Raab, M., et al. Immunity 32(4):541-556(2010). Lehmann, R., et al. Proteomics 9(23):5288-5295(2009). Fang, L., et al. Cell Cycle 8(17):2819-2827(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 115527074
• NCBI GeneID: 8631
• NCBI Accession #: NP_003717.3
• NCBI GenBank Nucleotide #: NM_003726.3
• UniProt Accession #: Q86WV1; O15268; D3DTV1
• Molecular Weight: 41,333 Da
• NCBI Official Full Name: src kinase-associated phosphoprotein 1 isoform 1
• NCBI Official Synonym Full Names: src kinase associated phosphoprotein 1
• NCBI Official Symbol: SKAP1
• NCBI Official Synonym Symbols: SCAP1; SKAP55; HEL-S-81p
• NCBI Protein Information: src kinase-associated phosphoprotein 1; pp55; SKAP-55; src family associated phosphoprotein 1; src family-associated phosphoprotein 1; src kinase-associated phosphoprotein of 55 kDa; epididymis secretory sperm binding protein Li 81p
• UniProt Protein Name: Src kinase-associated phosphoprotein 1
• Protein Family: Src kinase-associated phosphoprotein
• UniProt Gene Name: SKAP1
• UniProt Synonym Gene Names: SCAP1; SKAP55; SKAP-55; pp55
• UniProt Entry Name: SKAP1_HUMAN

NCBI Description

This gene encodes a T cell adaptor protein, a class of intracellular molecules with modular domains capable of recruiting additional proteins but that exhibit no intrinsic enzymatic activity. The encoded protein contains a unique N-terminal region followed by a PH domain and C-terminal SH3 domain. Along with the adhesion and degranulation-promoting adaptor protein, the encoded protein plays a critical role in inside-out signaling by coupling T-cell antigen receptor stimulation to the activation of integrins. [provided by RefSeq, Jul 2008]

Uniprot Description

SKAP55: Positively regulates T-cell receptor signaling by enhancing the MAP kinase pathway. Required for optimal conjugation between T-cells and antigen-presenting cells by promoting the clustering of integrin ITGAL on the surface of T-cells. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells. Belongs to the SKAP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: cytoplasm; plasma membrane; intercellular junction; nucleus

Molecular Function: protein binding; SH3/SH2 adaptor activity; protein complex binding; SH2 domain binding; SH3 domain binding; protein phosphatase binding; protein kinase binding

Biological Process: positive regulation of signal transduction; positive regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; T cell receptor signaling pathway