Human Neurocan ELISA Kit | NCAN elisa kit

Human Neurocan ELISA Kit

Cat. Num. AAA755302

• Gene Names: NCAN; CSPG3
• Reactivity: Human
• Synonyms: Neurocan; Human Neurocan ELISA Kit; NCAN elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 2.5-50ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This NCAN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human NCAN. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This NCAN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human NCAN. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NCAN elisa kit

Principle of the Assay: NCAN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NCAN antibody and an NCAN-HRP conjugate. The assay sample and buffer are incubated together with NCAN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NCAN concentration since NCAN from samples and NCAN-HRP conjugate compete for the anti-NCAN antibody binding site. Since the number of sites is limited, as more sites are occupied by NCAN from the sample, fewer sites are left to bind NCAN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NCAN concentration in each sample is interpolated from this standard curve.

Product Categories/Family for NCAN elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 118600983
• NCBI GeneID: 1463
• NCBI Accession #: NP_004377.2
• NCBI GenBank Nucleotide #: NM_004386.2
• Molecular Weight: 143,093 Da
• NCBI Official Full Name: neurocan core protein
• NCBI Official Synonym Full Names: neurocan
• NCBI Official Symbol: NCAN
• NCBI Official Synonym Symbols: CSPG3
• NCBI Protein Information: neurocan core protein; neurocan proteoglycan; chondroitin sulfate proteoglycan 3 (neurocan)
• UniProt Protein Name: Neurocan core protein
• Protein Family: Neurocan core protein
• UniProt Gene Name: NCAN
• UniProt Synonym Gene Names: CSPG3; NEUR
• UniProt Entry Name: NCAN_HUMAN

NCBI Description

Neurocan is a chondroitin sulfate proteoglycan thought to be involved in the modulation of cell adhesion and migration.[supplied by OMIM, Jul 2002]

Uniprot Description

CSPG3: May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid. Belongs to the aggrecan/versican proteoglycan family.

Protein type: Secreted; Cell adhesion; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p12

Cellular Component: proteinaceous extracellular matrix; lysosomal lumen; Golgi lumen; extracellular region

Molecular Function: extracellular matrix structural constituent; hyaluronic acid binding; calcium ion binding; carbohydrate binding

Biological Process: axon guidance; extracellular matrix organization and biogenesis; chondroitin sulfate biosynthetic process; central nervous system development; glycosaminoglycan metabolic process; pathogenesis; dermatan sulfate biosynthetic process; chondroitin sulfate metabolic process; chondroitin sulfate catabolic process; carbohydrate metabolic process; regulation of synapse structural plasticity; cell adhesion; skeletal development