G-CSFR / CD114 recombinant protein

Recombinant Human G-CSFR / CD114 Protein (His tag)

Cat. Num. AAA2546400

• Gene Names: CSF3R; SCN7; CD114; GCSFR
• Purity: > 85 % as determined by SDS-PAGE
• Synonyms: G-CSFR / CD114; Recombinant Human G-CSFR / CD114 Protein (His tag); CD114; GCSFR; CSF3R; G-CSFR / CD114 recombinant protein

• Host: Human Cells
• Purity/Purification: > 85 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 836

• Application Notes: The mature recombinant human GCSFR consists of 608 amino acids and predicts a molecular mass of 68 kDa by SDS-PAGE under reducing conditions, the apparent molecular mass of rhGCSFR is approximately 92 kDa due to glycosylation.
• Predicted N Terminal: Glu 25
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for G-CSFR / CD114 recombinant protein

Background: Granulocyte Colony Stimulating Factor Receptor (G-CSFR), also known as CD114, which belongs to the cytokine receptor superfamily, is a cell surface receptor for colony stimulating factor 3 (CSF3). It is a critical regulator of granulopoiesis. This type I membrane protein has a composite structure consisting of an immunoglobulin (Ig)-like domain, a cytokine receptor-homologous (CRH) domain and three fibronectin type III (FNIII) domains in the extracellular region. Mutations in the G-CSF receptor leading to carboxy-terminal truncation transduce hyperproliferative growth responses, and are implicated in the pathological progression of severe congenital neutropenia (SCN) to acute myelogenous leukemia (AML). Additionally, autocrine/paracrine stimulation of G-CSFR may be important in the biology of solid tumors, including metastasis.

Description: A DNA sequence encoding the extracellular domain (Met 1-Pro 621) of human G-CSF receptor (NP_000751.1) precursor was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 4503081
• NCBI GeneID: 1441
• NCBI Accession #: NP_000751.1
• NCBI GenBank Nucleotide #: NM_000760.3
• UniProt Accession #: Q99062
• Molecular Weight: 86,609 Da
• NCBI Official Full Name: granulocyte colony-stimulating factor receptor isoform a
• NCBI Official Synonym Full Names: colony stimulating factor 3 receptor
• NCBI Official Symbol: CSF3R
• NCBI Official Synonym Symbols: SCN7; CD114; GCSFR
• NCBI Protein Information: granulocyte colony-stimulating factor receptor
• UniProt Protein Name: Granulocyte colony-stimulating factor receptor
• UniProt Gene Name: CSF3R
• UniProt Synonym Gene Names: GCSFR; G-CSF receptor; G-CSF-R

NCBI Description

The protein encoded by this gene is the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. The encoded protein, which is a member of the family of cytokine receptors, may also function in some cell surface adhesion or recognition processes. Alternatively spliced transcript variants have been described. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia. [provided by RefSeq, Aug 2010]

Uniprot Description

GCSFR: the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. The encoded protein, which is a member of the family of cytokine receptors, may also function in some cell surface adhesion or recognition processes. Four transcript variant isoforms have been described.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein binding; receptor activity

Biological Process: defense response

Disease: Neutropenia, Severe Congenital, 7, Autosomal Recessive; Neutrophilia, Hereditary