SDS-Page
(15% SDS-PAGE (3ug))
Nedd8, 1-76 aa Recombinant Protein
Nedd8, 1-76 aa, Recombinant, Human (Neural Precursor Cell Expressed Developmentally Down-regulated 8, NEDD-8, MGC104393, MGC125896, MGC125897, NED8, Neddylin, Neural Precursor Cell Expressed Developmentally Down-regulated Gene 8, Rub1, Ubiquitin-like Prot
Reactivity
Human
Purity
Highly Purified95% by SDS-PAGE
Synonyms
Nedd8; 1-76 aa; Recombinant; Human (Neural Precursor Cell Expressed Developmentally Down-regulated 8; NEDD-8; MGC104393; MGC125896; MGC125897; NED8; Neddylin; Neural Precursor Cell Expressed Developmentally Down-regulated Gene 8; Rub1; Ubiquitin-like Prot; 1-76 aa recombinant protein
Host
E Coli
Reactivity
Human
Purity/Purification
Highly Purified
95% by SDS-PAGE
95% by SDS-PAGE
Form/Format
Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, 0.5mM DTT, 50mM sodium chloride, 10% glycerol.
Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMLI KVKTLTGKEI EIDIEPTDKV ERIKERVEEK EGIPPQQQRL IYSGKQMNDE
KTAADYKILG GSVLHLVLAL RGG
KTAADYKILG GSVLHLVLAL RGG
Endotoxin Level
1.0 EU per 1 ug of protein (determined by LAL method)
Preparation and Storage
For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SDS-Page
(15% SDS-PAGE (3ug))
SDS-Page
(15% SDS-PAGE (3ug))
Related Product Information for Nedd8, 1-76 aa recombinant protein
NEDD8 belongs to the ubiquitin family and human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The NEDD8 system is essential for the regulation of protein degradation pathways involved in cell cycle progression, morphogenesis and tumorigenesis. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. Recombinant human NEDD8 protein was expressed in E. coli and purified by using conventional chromatography techniques, after refolding of the isolated inclusion body in renaturation buffer.
Product Categories/Family for Nedd8, 1-76 aa recombinant protein
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Product Notes
The Nedd8, 1-76 aa (Catalog #AAA636543) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The Nedd8, 1-76 aa, Recombinant, Human (Neural Precursor Cell Expressed Developmentally Down-regulated 8, NEDD-8, MGC104393, MGC125896, MGC125897, NED8, Neddylin, Neural Precursor Cell Expressed Developmentally Down-regulated Gene 8, Rub1, Ubiquitin-like Prot reacts with Human and may cross-react with other species as described in the data sheet. The amino acid sequence is listed below: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMLI KVKTLTGKEI EIDIEPTDKV ERIKERVEEK EGIPPQQQRL IYSGKQMNDE KTAADY KILG GSVLHLVLAL RGG. It is sometimes possible for the material contained within the vial of "Nedd8, 1-76 aa, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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