Western Blot (WB)
Rabbit anti-Human MLK-3 (Mixed lineage kinase-3) Polyclonal Antibody | anti-MLK 3 antibody
Anti-MLK-3 (Mixed lineage kinase-3) Antibody
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified
Synonyms
MLK-3 (Mixed lineage kinase-3); Polyclonal Antibody; Anti-MLK-3 (Mixed lineage kinase-3) Antibody; anti-MLK 3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified
Form/Format
Liquid
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-MLK 3 antibody
Western Blot (WB), ELISA (EIA)
Immunogen
Ni-NTA purified recombinant human MLK-3 expressed in E Coli strain M15.
Storage Buffer
PBS, pH 7.4 with 0.05% sodium azide.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Western Blot (WB)
Western Blot (WB)
Related Product Information for anti-MLK 3 antibody
Mixed lineage kinase-3 (MLK-3) is a 97 kDa serine/threonine kinase with multiple interaction domains, including a Cdc42 binding motif, but unknown function. In support of this role, we demonstrate that MLK-3 can specifically activate the SAPK/JNK and p38/RK pathways, but has no effect on the activation of ERKs. Immunoprecipitated MLK-3 catalyzed the phosphorylation of SEK1 in vitro, and co-transfected MLK-3 induced phosphorylation of SEK1 and MKK3 at sites required for activation, suggesting direct regulation of these protein kinases. Furthermore, interactions between MLK-3 and SEK and MLK-3 and MKK6 were observed in co-precipitation experiments. Finally, kinase-dead mutants of MLK-3 blocked activation of the SAPK pathway by a newly identified mammalian analog of Ste20, germinal center kinase, but not by MEKK, suggesting that MLK-3 functions to activate the SAPK/JNK and p38/RK cascades in response to stimuli transduced by Ste20-like kinases.
References
1. Rana, A. Gallo, K. Godowski, P. Hirai, S., Ohno, S. Zon, L., Kyriakis, J.M. and Avruch, J. The mixed lineage kinase SPRK phosphorylates and activates the stress-activated protein kinase activator, SEK-1. J. Biol. Chem. 1996. 271, 19025.
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Product Notes
The MLK 3 (Catalog #AAA418213) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MLK-3 (Mixed lineage kinase-3) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MLK-3 (Mixed lineage kinase-3) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). Researchers should empirically determine the suitability of the MLK 3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MLK-3 (Mixed lineage kinase-3), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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