CSF3R/G-CSFR ELISA Pair Set | CSF3R elisa pair set

CSF3R/G-CSFR Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118215

• Synonyms: CSF3R/G-CSFR; CSF3R/G-CSFR Matched ELISA Antibody Pair Set; Human; Human G-CSF R/CD114/CSF3R ELISA Pair Set; colony stimulating factor 3 receptor (granulocyte); CD114 Matched ELISA Antibody Pair Set; CSF3R Matched ELISA Antibody Pair Set; G-CSF R Matched ELISA Antibody Pair Set; GCSFR Matched ELISA Antibody Pair Set; CSF3R elisa pair set

• Specificity: Quantitative determination of Human CSF3R/G-CSFR

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 50-3200pg/mL
• Sensitivity: 50pg/ml
• Materials Provided: 1. Capture Antibody 0.5 mg/mL of mouse anti-Human G-CSF R/CD114/CSF3R monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL of mouse anti-Human G-CSF R/CD114/CSF3R monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use. ; 3. Standard Each vial contains 215 ng of recombinant Human G-CSF R/CD114/CSF3R. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3200 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for CSF3R elisa pair set

Granulocyte Colony Stimulating Factor Receptor (G-CSFR), also known as CD114, which belongs to the cytokine receptor superfamily, is a cell surface receptor for colony stimulating factor 3 (CSF3). It is a critical regulator of granulopoiesis. This type I membrane protein has a composite structure consisting of an immunoglobulin(Ig)-like domain, a cytokine receptor-homologous (CRH) domain and three fibronectin type III (FNIII) domains in the extracellular region. Mutations in the G-CSF receptor leading to carboxy-terminal truncation transduce hyperproliferative growth responses, and are implicated in the pathological progression of severe congenital neutropenia (SCN) to acute myelogenous leukemia (AML). Additionally, autocrine/paracrine stimulation of G-CSFR may be important in the biology of solid tumors, including metastasis.

This CSF3R/G-CSFR Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human CSF3R/G-CSFR. It contains Human CSF3R/G-CSFR capture antibody, Human CSF3R/G-CSFR detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human CSF3R/G-CSFR protein.

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AKT Signaling Pathway

References

Kasper B, et al. (1999) Association of src-kinase Lyn and non-src-kinase Syk with the granulocyte colony-stimulating factor receptor (G-CSFR) is not abrogated in neutrophils from severe congenital neutropenia patients with point mutations in the G-CSFR mRNA. Int J Hematol. 70(4): 241-7.Hollenstein U, et al. (2000) Endotoxin down-modulates granulocyte colony-stimulating factor receptor (CD114) on human neutrophils. J Infect Dis. 182(1): 343-6.Kindwall-Keller TL, et al. (2008) Role of the proteasome in modulating native G-CSFR expression. Cytokine. 43(2): 114-23.Beel K, et al. (2009) G-CSF receptor (CSF3R) mutations in X-linked neutropenia evolving to acute myeloid leukemia or myelodysplasia. Haematologica. 94(10): 1449-52.