Immunofluorescence (IF)
(Confocal immunofluorescence analysis of Human GCSFR in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human GCSFR monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody, countstained with Alexa Fluor 546-conjugated phallotoxins (red) and DAPI (blue). Positive staining was localized to cytoplasm and cytomembrane.)
Mouse anti-Human CSF3R/G-CSFR Monoclonal Antibody | anti-CSF3R antibody
Anti-CSF3R/G-CSFR Antibody, Mouse Monoclonal
ELISA (Cap): 1:250-1:2000
ELISA (Cap): This antibody will detect Human G-CSF R/CD114/CSF3R in ELISA pair set.
In a sandwich ELISA, it can be used as capture antibody when paired with.
Immunofluorescence (IF)
(Confocal immunofluorescence analysis of Human GCSFR in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human GCSFR monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody, countstained with Alexa Fluor 546-conjugated phallotoxins (red) and DAPI (blue). Positive staining was localized to cytoplasm and cytomembrane.)
Immunofluorescence (IF)
(Confocal immunofluorescence analysis of Human GCSFR in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human GCSFR monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody, countstained with Alexa Fluor 546-conjugated phallotoxins (red) and DAPI (blue). Positive staining was localized to cytoplasm and cytomembrane.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. The encoded protein, which is a member of the family of cytokine receptors, may also function in some cell surface adhesion or recognition processes. Alternatively spliced transcript variants have been described. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia. [provided by RefSeq, Aug 2010]
Uniprot Description
GCSFR: the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. The encoded protein, which is a member of the family of cytokine receptors, may also function in some cell surface adhesion or recognition processes. Four transcript variant isoforms have been described.
Protein type: Receptor, cytokine; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1p35-p34.3
Cellular Component: integral to plasma membrane; extracellular region
Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; receptor activity
Biological Process: neutrophil chemotaxis; cytokine and chemokine mediated signaling pathway; defense response; signal transduction; cell adhesion
Disease: Neutrophilia, Hereditary
Research Articles on CSF3R
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Product Notes
The CSF3R csf3r (Catalog #AAA8101808) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-CSF3R/G-CSFR Antibody, Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CSF3R/G-CSFR can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA) (Cap), Immunocytochemistry (ICC), Immunofluorescence (IF). ICC/IF: 1:20-1:100 ELISA (Cap): 1:250-1:2000 ELISA (Cap): This antibody will detect Human G-CSF R/CD114/CSF3R in ELISA pair set. In a sandwich ELISA, it can be used as capture antibody when paired with. Researchers should empirically determine the suitability of the CSF3R csf3r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CSF3R/G-CSFR, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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