Mouse Cartilage acidic protein 1 ELISA Kit | Crtac1 elisa kit

Mouse Cartilage acidic protein 1 ELISA Kit

Cat. Num. AAA764804

• Gene Names: CRTAC1; ASPIC; ASPIC1; CEP-68
• Reactivity: Mouse
• Synonyms: Cartilage acidic protein 1; Mouse Cartilage acidic protein 1 ELISA Kit; Crtac1/Aspic1/Cep68/Protein CRTAC1-B/68 kDa chondrocyte-expressed protein/CEP-68/ASPIC; Crtac1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection ofCRP. No significant crossreactivity or interference betweenCRP and analogues was observed.
• Sequence Length: 661

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.625-40ng/ml
• Sensitivity: <0.375ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high levelCRP were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high levelCRP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Crtac1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-CRP antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CRP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CRP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofCRP can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 47777317
• NCBI GeneID: 55118
• NCBI Accession #: NP_060528.3
• NCBI GenBank Nucleotide #: NM_018058.6
• Molecular Weight: 68,059 Da
• NCBI Official Full Name: cartilage acidic protein 1 isoform A
• NCBI Official Synonym Full Names: cartilage acidic protein 1
• NCBI Official Symbol: CRTAC1
• NCBI Official Synonym Symbols: ASPIC; ASPIC1; CEP-68
• NCBI Protein Information: cartilage acidic protein 1
• UniProt Protein Name: Cartilage acidic protein 1
• Protein Family: Cartilage acidic protein
• UniProt Gene Name: CRTAC1
• UniProt Synonym Gene Names: ASPIC1; CEP68; CEP-68
• UniProt Entry Name: CRAC1_HUMAN

NCBI Description

This gene encodes a glycosylated extracellular matrix protein that is found in the interterritorial matrix of articular deep zone cartilage. This protein is used as a marker to distinguish chondrocytes from osteoblasts and mesenchymal stem cells in culture. The presence of FG-GAP motifs and an RGD integrin-binding motif suggests that this protein may be involved in cell-cell or cell-matrix interactions. Copy number alterations in this gene have been observed in neurofibromatosis type 1-associated glomus tumors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]

Uniprot Description

CRTAC1: a glycosylated extracellular matrix protein that is found in the interterritorial matrix of articular deep zone cartilage. This protein is used as a marker to distinguish chondrocytes from osteoblasts and mesenchymal stem cells in culture. The presence of FG-GAP motifs and an RGD integrin-binding motif suggests that this protein may be involved in cell-cell or cell-matrix interactions. Copy number alterations in this gene have been observed in neurofibromatosis type 1-associated glomus tumors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 10q22

Cellular Component: growth cone; proteinaceous extracellular matrix

Molecular Function: calcium ion binding; protein binding

Biological Process: axonal fasciculation; olfactory bulb development