Typical Testing Data/Standard Curve (for reference only)
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Canine Cartilage acidic protein 1 (CRTAC1) ELISA Kit | CRTAC1 elisa kit
Canine Cartilage acidic protein 1 (CRTAC1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Principle of the Assay: CRTAC1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CRTAC1 antibody and an CRTAC1-HRP conjugate. The assay sample and buffer are incubated together with CRTAC1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CRTAC1 concentration since CRTAC1 from samples and CRTAC1-HRP conjugate compete for the anti-CRTAC1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CRTAC1 from the sample, fewer sites are left to bind CRTAC1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CRTAC1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a glycosylated extracellular matrix protein that is found in the interterritorial matrix of articular deep zone cartilage. This protein is used as a marker to distinguish chondrocytes from osteoblasts and mesenchymal stem cells in culture. The presence of FG-GAP motifs and an RGD integrin-binding motif suggests that this protein may be involved in cell-cell or cell-matrix interactions. Copy number alterations in this gene have been observed in neurofibromatosis type 1-associated glomus tumors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]
Uniprot Description
CRTAC1: a glycosylated extracellular matrix protein that is found in the interterritorial matrix of articular deep zone cartilage. This protein is used as a marker to distinguish chondrocytes from osteoblasts and mesenchymal stem cells in culture. The presence of FG-GAP motifs and an RGD integrin-binding motif suggests that this protein may be involved in cell-cell or cell-matrix interactions. Copy number alterations in this gene have been observed in neurofibromatosis type 1-associated glomus tumors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 10q22
Cellular Component: proteinaceous extracellular matrix; growth cone
Molecular Function: calcium ion binding
Biological Process: olfactory bulb development; axonal fasciculation
Research Articles on CRTAC1
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Product Notes
The Canine CRTAC1 crtac1 (Catalog #AAA7229762) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7229762 ELISA Kit recognizes Canine CRTAC1. It is sometimes possible for the material contained within the vial of "Cartilage acidic protein 1 (CRTAC1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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