Rabbit anti-Human, Mouse GPD1L Polyclonal Antibody | anti-GPD1L antibody

GPD1L, NT (GPD1L, KIAA0089, Glycerol-3-phosphate dehydrogenase 1-like protein) (AP)

Cat. Num. AAA6303491

• Gene Names: GPD1L; GPD1-L
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GPD1L; Polyclonal Antibody; NT (GPD1L; KIAA0089; Glycerol-3-phosphate dehydrogenase 1-like protein) (AP); Glycerol-3-phosphate dehydrogenase 1-like protein; anti-GPD1L antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-GPD1L antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: GPD1L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 47-77 amino acids from the N-terminal region of human GPD1L.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(GPD1L Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the GPD1L antibody detected the GPD1L protein (arrow).)

Western Blot (WB)

(GPD1L Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the GPD1L antibody detected the GPD1L protein (arrow).)

Immunohistochemistry (IHC)

(GPD1L antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GPD1L antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(GPD1L Antibody (N-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-GPD1L antibody

The protein encoded by this gene catalyzes the conversion of sn-glycerol 3-phosphate to glycerone phosphate. The encoded protein is found in the cytoplasm, associated with the plasma membrane, where it binds the sodium channel, voltage-gated, type V, alpha subunit (SCN5A). Defects in this gene are a cause of Brugada syndrome type 2 (BRS2) as well as sudden infant death syndrome (SIDS).

Product Categories/Family for anti-GPD1L antibody

Antibodies; KIAA; orf

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Liu, M., et al. Circ. Res. 105(8):737-745(2009). Valdivia, C.R., et al. Am. J. Physiol. Heart Circ. Physiol. 297 (4), H1446-H1452 (2009): Makiyama, T., et al. Circ. J. 72(10):1705-1706(2008). London, B., et al. Circulation 116(20):2260-2268(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 24307999
• NCBI GeneID: 23171
• NCBI Accession #: NP_055956.1
• NCBI GenBank Nucleotide #: NM_015141.3
• UniProt Accession #: Q8N335; Q14702; Q9BRM5; A8K9U3
• Molecular Weight: 38,419 Da
• NCBI Official Full Name: glycerol-3-phosphate dehydrogenase 1-like protein
• NCBI Official Synonym Full Names: glycerol-3-phosphate dehydrogenase 1-like
• NCBI Official Symbol: GPD1L
• NCBI Official Synonym Symbols: GPD1-L
• NCBI Protein Information: glycerol-3-phosphate dehydrogenase 1-like protein
• UniProt Protein Name: Glycerol-3-phosphate dehydrogenase 1-like protein
• Protein Family: Glycerol-3-phosphate dehydrogenase 1-like protein
• UniProt Gene Name: GPD1L
• UniProt Synonym Gene Names: KIAA0089; GPD1-L
• UniProt Entry Name: GPD1L_HUMAN

NCBI Description

The protein encoded by this gene catalyzes the conversion of sn-glycerol 3-phosphate to glycerone phosphate. The encoded protein is found in the cytoplasm, associated with the plasma membrane, where it binds the sodium channel, voltage-gated, type V, alpha subunit (SCN5A). Defects in this gene are a cause of Brugada syndrome type 2 (BRS2) as well as sudden infant death syndrome (SIDS). [provided by RefSeq, Jul 2010]

Uniprot Description

GPD1L: Plays a role in regulating cardiac sodium current; decreased enzymatic activity with resulting increased levels of glycerol 3-phosphate activating the DPD1L-dependent SCN5A phosphorylation pathway, may ultimately lead to decreased sodium current; cardiac sodium current may also be reduced due to alterations of NAD(H) balance induced by DPD1L. Defects in GPD1L are the cause of Brugada syndrome type 2 (BRGDA2). An autosomal dominant tachyarrhythmia characterized by right bundle branch block and ST segment elevation on an electrocardiogram (ECG). It can cause the ventricles to beat so fast that the blood is prevented from circulating efficiently in the body. When this situation occurs (called ventricular fibrillation), the individual will faint and may die in a few minutes if the heart is not reset. Defects in GPD1L are a cause of sudden infant death syndrome (SIDS). SIDS is the sudden death of an infant younger than 1 year that remains unexplained after a thorough case investigation, including performance of a complete autopsy, examination of the death scene, and review of clinical history. Pathophysiologic mechanisms for SIDS may include respiratory dysfunction, cardiac dysrhythmias, cardiorespiratory instability, and inborn errors of metabolism, but definitive pathogenic mechanisms precipitating an infant sudden death remain elusive. Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.

Protein type: EC 1.1.1.8; Oxidoreductase; Lipid Metabolism - glycerophospholipid

Chromosomal Location of Human Ortholog: 3p22.3

Cellular Component: glycerol-3-phosphate dehydrogenase complex; plasma membrane; cytosol

Molecular Function: glycerol-3-phosphate dehydrogenase (NAD+) activity; protein homodimerization activity; sodium channel regulator activity; NAD binding

Biological Process: NADH metabolic process; regulation of heart rate; NAD metabolic process; phospholipid metabolic process; glycerophospholipid biosynthetic process; carbohydrate metabolic process; negative regulation of peptidyl-serine phosphorylation; phosphatidic acid biosynthetic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; glycerol-3-phosphate catabolic process

Disease: Brugada Syndrome 2