Immunohistochemistry (IHC)
(Immunohistochemistry of whole-mount transverse sections of E12.5 mouse embryos using MBS622480. Incubated for 1 hour at RT. The signal is confined to areas in different tissues with mesodermal lineages.)
Rabbit Wnt3a Polyclonal Antibody | anti-WNT3A antibody
Wnt3a (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member)
Purified by affinity chromatography.
Purified by affinity chromatography.
Dilution: Immunohistochemistry (Paraffin): 1:200 stains whole-mount transverse section of E12.5 mouse embryos. Incubated with diluted W3011-02B for 1 hour at RT. Tissue was pretreated with no heat induced epitope retrieval, citrate pH 6.
Western Blot: 1:1000. Rat whole brain lysate was resolved by electrophoresis and transferred to PVDF. Proteins were visualized using an IgG (HRP) Dk xRb and a chemiluminescence detection system.
Immunohistochemistry (IHC)
(Immunohistochemistry of whole-mount transverse sections of E12.5 mouse embryos using MBS622480. Incubated for 1 hour at RT. The signal is confined to areas in different tissues with mesodermal lineages.)
Immunohistochemistry (IHC)
(Immunohistochemistry of whole-mount transverse sections of E12.5 mouse embryos using MBS622480. Incubated for 1 hour at RT. The signal is confined to areas in different tissues with mesodermal lineages.)
Western Blot (WB)
(Western Blot: Rat whole brain lysate (lane 1) and human recombinant Wnt3A protein (lane 2) were resolved by SDS-PAGE, transferred to PVDF and probed with MBS622480 (1:1000). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescent detection system. Arrow indicates Wnt3A (~40kD).)
Western Blot (WB)
(Western Blot: Rat whole brain lysate (lane 1) and human recombinant Wnt3A protein (lane 2) were resolved by SDS-PAGE, transferred to PVDF and probed with MBS622480 (1:1000). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescent detection system. Arrow indicates Wnt3A (~40kD).)
NCBI and Uniprot Product Information
NCBI Description
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq]
Uniprot Description
WNT3A: Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. Interacts with PORCN. Interacts with APCDD1 and WLS. Component of the Wnt-Fzd-LRP5-LRP6 signaling complex that contains a WNT protein, a FZD protein and LRP5 or LRP6. Interacts directly in the complex with LRP6. Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate. Belongs to the Wnt family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 1q42
Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; early endosome membrane; Golgi lumen; endoplasmic reticulum lumen; plasma membrane; extracellular region
Molecular Function: protein domain specific binding; protein binding; frizzled binding; transcription coactivator activity; receptor agonist activity; frizzled-2 binding
Biological Process: positive regulation of mesodermal cell fate specification; axon guidance; extracellular matrix organization and biogenesis; positive regulation of protein binding; cell proliferation in forebrain; positive regulation of transcription, DNA-dependent; positive regulation of receptor internalization; positive regulation of caspase activity; palate development; positive regulation of collateral sprouting in the absence of injury; Wnt receptor signaling pathway through beta-catenin; negative regulation of axon extension involved in axon guidance; Wnt receptor signaling pathway in forebrain neuroblast division; negative regulation of neurogenesis; neuron differentiation; mammary gland development; positive regulation of cell proliferation; positive regulation of B cell proliferation; hemopoiesis; heart looping; dorsoventral neural tube patterning; inner ear morphogenesis; in utero embryonic development; hippocampus development; negative regulation of fat cell differentiation; positive regulation of peptidyl-serine phosphorylation; osteoblast differentiation; paraxial mesodermal cell fate commitment; signalosome assembly; spinal cord association neuron differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; positive regulation of protein amino acid phosphorylation
Research Articles on WNT3A
Similar Products
Product Notes
The WNT3A wnt3a (Catalog #AAA622480) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Wnt3a (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Wnt3a can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Immunohistochemistry and Western Blot. Dilution: Immunohistochemistry (Paraffin): 1:200 stains whole-mount transverse section of E12.5 mouse embryos. Incubated with diluted W3011-02B for 1 hour at RT. Tissue was pretreated with no heat induced epitope retrieval, citrate pH 6. Western Blot: 1:1000. Rat whole brain lysate was resolved by electrophoresis and transferred to PVDF. Proteins were visualized using an IgG (HRP) Dk xRb and a chemiluminescence detection system. Researchers should empirically determine the suitability of the WNT3A wnt3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Wnt3a, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
