Bovine C-reactive protein, CRP ELISA Kit | CRP elisa kit

Bovine C-reactive protein, CRP ELISA Kit

Cat. Num. AAA700792

• Gene Names: CRP; PTX1
• Reactivity: Bovine
• Synonyms: C-reactive protein; CRP; Bovine C-reactive protein; CRP ELISA Kit; CRP elisa kit

• Reactivity: Bovine
• Sequence Length: 224

• Samples: Serum, plasma
• Detection Range: 1.25 ng/ml-80 ng/ml
• Sensitivity: 1.25 ng/ml
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CRP elisa kit

Introduction: C-reactive protein (CRP) is a protein found in the blood in response to inflammation (an acute-phase protein). CRP is synthesized by the liver in response to factors released by fat cells (adipocytes). It is a member of the pentraxin family of proteins. CRP is a member of the class of acute-phase reactants as its levels rise dramatically during inflammatory processes occurring in the body. This increment is due to a rise in the plasma concentration of IL-6, which is produced predominantly by macrophages as well as adipocytes. CRP binds to phosphocholine on microbes. It is thought to assist in complement binding to foreign and damaged cells and enhances phagocytosis by macrophages, which express a receptor for CRP. It is also believed to play another important role in innate immunity, as an early defense system against infections. CRP rises up to 50,000-fold in acute inflammation, such as infection. It rises above normal limits within 6 hours, and peaks at 48 hours. Its half-life is constant, and therefore its level is mainly determined by the rate of production (and hence the severity of the precipitating cause). Serum amyloid A is a related acute-phase marker that responds rapidly in similar circumstances.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CRP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for CRP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain CRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CRP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 17975775
• NCBI GeneID: 1401
• UniProt Accession #: P02741; Q08AK3; Q8WW75; A8K078; D3DVD9; D3DVE0
• Molecular Weight: 10,415 Da
• NCBI Official Full Name: CRP
• NCBI Official Synonym Full Names: C-reactive protein, pentraxin-related
• NCBI Official Symbol: CRP
• NCBI Official Synonym Symbols: PTX1
• NCBI Protein Information: C-reactive protein; pentraxin 1
• UniProt Protein Name: C-reactive protein
• Protein Family: Cryparin
• UniProt Gene Name: CRP
• UniProt Synonym Gene Names: PTX1
• UniProt Entry Name: CRP_HUMAN

NCBI Description

The protein encoded by this gene belongs to the pentaxin family. It is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood. Consequently, the level of this protein in plasma increases greatly during acute phase response to tissue injury, infection, or other inflammatory stimuli. [provided by RefSeq, Sep 2009]

Uniprot Description

Function: Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

Cofactor: Binds 2 calcium ions per subunit.