Human TAFI ELISA Kit | CPB2 elisa kit

Human TAFI ELISA Kit

Cat. Num. AAA8248347

• Gene Names: CPB2; CPU; PCPB; TAFI
• Reactivity: Human
• Synonyms: TAFI; Human TAFI ELISA Kit; Carboxypeptidase B2; Carboxypeptidase U; CPU; Plasma carboxypeptidase B; pCPB; Thrombin-activable fibrinolysis inhibitor; CPB2 elisa kit

• Reactivity: Human
• Specificity: The Human TAFI ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human TAFI proteins within the range of 156 pg/ml - 10000 pg/ml.
• Sequence Length: 386

• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: <10 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CPB2 elisa kit

Principle of the Assay: The Human TAFI ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TAFI in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Human TAFI coated on a 96-well plate. Standards and samples are pipetted into the wells and TAFI present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TAFI antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TAFI bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Carboxypeptidase B2 (CPB2), also known as carboxypeptidase U (CPU), plasma carboxypeptidase B (pCPB) or thrombin-activatable fibrinolysis inhibitor (TAFI), is an enzyme that, in humans, is encoded by the gene CPB2. CPB2 is synthesized by the liver and circulates in the plasma as a plasminogen-bound zymogen. When it is activated by proteolysis at residue Arg92 by the thrombin/thrombomodulin complex, CPB2 exhibits carboxypeptidase activity. Activated CPB2 reduces fibrinolysis by removing the fibrin C-terminal residues that are important for the binding and activation of plasminogen.

NCBI and Uniprot Product Information

• NCBI GI #: 513126877
• NCBI GeneID: 1361
• NCBI Accession #: NP_001265470.1
• NCBI GenBank Nucleotide #: NM_001278541.1
• UniProt Accession #: Q96IY4; Q15114; Q5T9K1; Q5T9K2; Q9P2Y6; A8K464
• Molecular Weight: 40,922 Da
• NCBI Official Full Name: carboxypeptidase B2 isoform 2 preproprotein
• NCBI Official Synonym Full Names: carboxypeptidase B2
• NCBI Official Symbol: CPB2
• NCBI Official Synonym Symbols: CPU; PCPB; TAFI
• NCBI Protein Information: carboxypeptidase B2
• UniProt Protein Name: Carboxypeptidase B2
• Protein Family: Carboxypeptidase
• UniProt Gene Name: CPB2
• UniProt Synonym Gene Names: CPU; pCPB; TAFI
• UniProt Entry Name: CBPB2_HUMAN

NCBI Description

Carboxypeptidases are enzymes that hydrolyze C-terminal peptide bonds. The carboxypeptidase family includes metallo-, serine, and cysteine carboxypeptidases. According to their substrate specificity, these enzymes are referred to as carboxypeptidase A (cleaving aliphatic residues) or carboxypeptidase B (cleaving basic amino residues). The protein encoded by this gene is activated by trypsin and acts on carboxypeptidase B substrates. After thrombin activation, the mature protein downregulates fibrinolysis. Polymorphisms have been described for this gene and its promoter region. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Uniprot Description

CPB2: Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down- regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.17.20; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 13q14.11

Biological Process: fibrinolysis