Rabbit anti-Human TYR Polyclonal Antibody | anti-TYR antibody

TYR, ID (Tyrosinase, Monophenol Monooxygenase, Tumor Rejection Antigen AB, SK29-AB, LB24-AB) (Biotin)

Cat. Num. AAA6504278

• Gene Names: TYR; ATN; CMM8; OCA1; OCA1A; OCAIA; SHEP3
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Purified by Ammonium Sulfate Precipitation
• Synonyms: TYR; Polyclonal Antibody; ID (Tyrosinase; Monophenol Monooxygenase; Tumor Rejection Antigen AB; SK29-AB; LB24-AB) (Biotin); anti-TYR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Tyrosinase.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-TYR antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide corresponding to a region between aa183-213 from the center region of human Tyrosinase, UniProt Accession #NP_000363, P14679 Cellular Localization: Melanosome membrane; single-pass, type 1 membrane protein
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of Tyrosinase Antibody (Center) in A375, 293 cell line lysates (35ug/lane). Tyrosinase (arrow) was detected using the purified Pab.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of TYR Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Immunohistochemistry (IHC)

(TYR Antibody (Center) (AP7345c)immunohistochemistry analysis in formalin fixed and paraffin embedded human melanoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TYR Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-TYR antibody

TYR catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The protein has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this protein result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation.

Product Categories/Family for anti-TYR antibody

Antibodies; Disease Markers

References

1. Ostankovitch, M. J. Immunol. 182(8), 4830-4835 (2009). 2. Chintamaneni, C.D. Proc. Natl. Acad. Sci. USA 88(12), 5272-5276 (1991).

NCBI and Uniprot Product Information

• NCBI GI #: 4507753
• NCBI GeneID: 7299
• NCBI Accession #: NP_000363
• NCBI GenBank Nucleotide #: NM_000372
• UniProt Accession #: P14679; Q15675; Q15676; Q15680; Q8TAK4; Q9BYY0; Q9BZX1
• Molecular Weight: 42,914 Da
• NCBI Official Full Name: tyrosinase
• NCBI Official Synonym Full Names: tyrosinase
• NCBI Official Symbol: TYR
• NCBI Official Synonym Symbols: ATN; CMM8; OCA1; OCA1A; OCAIA; SHEP3
• NCBI Protein Information: tyrosinase; LB24-AB; SK29-AB; monophenol monooxygenase; oculocutaneous albinism IA; tumor rejection antigen AB
• UniProt Protein Name: Tyrosinase
• Protein Family: Tyrosinase
• UniProt Gene Name: TYR
• UniProt Entry Name: TYRO_HUMAN

Uniprot Description

TYR: This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone. Increased expression after UVB irradiation. Belongs to the tyrosinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; EC 1.14.18.1; Cofactor and Vitamin Metabolism - riboflavin; Amino Acid Metabolism - tyrosine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q14.3

Cellular Component: perinuclear region of cytoplasm; lysosome; melanosome membrane; cytoplasm; melanosome; integral to membrane; Golgi-associated vesicle

Molecular Function: protein binding; protein homodimerization activity; copper ion binding; protein heterodimerization activity; monophenol monooxygenase activity

Biological Process: cell proliferation; visual perception; thymus development; melanin biosynthetic process from tyrosine; eye pigment biosynthetic process

Disease: Albinism, Ocular, With Sensorineural Deafness; Albinism, Oculocutaneous, Type Ib; Albinism, Oculocutaneous, Type Ia; Skin/hair/eye Pigmentation, Variation In, 3