Chicken Interleukin-36 beta ELISA Kit | IL-36 beta/ IL-1F8/FIL-1h/IL-1H2 elisa kit

Chicken Interleukin-36 beta ELISA Kit

Cat. Num. AAA752883

• Gene Names: IL36B; FIL1; FIL1H; IL1F8; IL1H2; IL-1F8; IL-1H2; IL1-ETA; FIL1-(ETA); FILI-(ETA)
• Reactivity: Chicken
• Synonyms: Interleukin-36 beta; Chicken Interleukin-36 beta ELISA Kit; IL-36 beta/ IL-1F8/FIL-1h/IL-1H2 elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Intended Uses: This IL-36b ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken IL-36b. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This IL-36b ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken IL-36b. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-36 beta/ IL-1F8/FIL-1h/IL-1H2 elisa kit

Principle of the Assay: IL-36b ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-36b antibody and an IL-36b-HRP conjugate. The assay sample and buffer are incubated together with IL-36b-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-36b concentration since IL-36b from samples and IL-36b-HRP conjugate compete for the anti-IL-36b antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-36b from the sample, fewer sites are left to bind IL-36b-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-36b concentration in each sample is interpolated from this standard curve.

Product Categories/Family for IL-36 beta/ IL-1F8/FIL-1h/IL-1H2 elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 27894313
• NCBI GeneID: 27177
• NCBI Accession #: NP_775270.1
• NCBI GenBank Nucleotide #: NM_173178.2
• Molecular Weight: 17,702 Da
• NCBI Official Full Name: interleukin-36 beta isoform 2
• NCBI Official Synonym Full Names: interleukin 36, beta
• NCBI Official Symbol: IL36B
• NCBI Official Synonym Symbols: FIL1; FIL1H; IL1F8; IL1H2; IL-1F8; IL-1H2; IL1-ETA; FIL1-(ETA); FILI-(ETA)
• NCBI Protein Information: interleukin-36 beta; FIL1 eta; IL-1 eta; IL-1F8 (FIL1-eta); interleukin-1 eta; interleukin 1, eta; interleukin-1 homolog 2; Interleukin-1 Superfamily e; family of interleukin 1-eta; interleukin-1 family member 8; IL1F8 (Canonical product IL-1F8a); interleukin 1 family, member 8 (eta)
• UniProt Protein Name: Interleukin-36 beta
• Protein Family: Interleukin
• UniProt Gene Name: IL36B
• UniProt Synonym Gene Names: IL1F8; IL1H2; IL-1 eta; IL-1F8; IL-1H2
• UniProt Entry Name: IL36B_HUMAN

NCBI Description

The protein encoded by this gene is a member of the interleukin 1 cytokine family. Protein structure modeling indicated that this cytokine may contain a 12-stranded beta-trefoil structure that is conserved between IL1A (IL-A alpha) and IL1B (IL-1 beta). This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Two alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

IL36B: Stimulates production of interleukin-6 and interleukin-8 in synovial fibrobasts, articular chondrocytes and mature adipocytes. Induces expression of a number of antimicrobial peptides including beta-defensins 4 and 103 as well as a number of matrix metalloproteases. Belongs to the IL-1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 2q14

Cellular Component: extracellular space; extracellular region

Molecular Function: interleukin-1 receptor binding; cytokine activity

Biological Process: positive regulation of T cell differentiation; cytokine and chemokine mediated signaling pathway; positive regulation of interleukin-6 production; innate immune response; immune response; inflammatory response