Carboxypeptidase B2 Recombinant Protein | CPB2 recombinant protein

Carboxypeptidase B2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120832

• Gene Names: CPB2; CPU; PCPB; TAFI
• Purity: >97% as determined by SDS-PAGE
• Synonyms: Carboxypeptidase B2; Carboxypeptidase B2 Protein; Human; Recombinant (His Tag); Human Carboxypeptidase B2/CPB2 Protein (His Tag); CPU Protein; PCPB Protein; TAFI Protein; carboxypeptidase B2 (plasma); CPB2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Val423

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Phe 23
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human CPB2 (NP_001863.2) extracellular domain (Met 1-Val 423) was expressed, fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CPB2 recombinant protein

Background: Carboxypeptidase B2, also known as Carboxypeptidase U, Thrombin-activable fibrinolysis inhibitor, Plasma carboxypeptidase B, CPB2, is a secreted protein which belongs to the peptidase M14 family. Carboxypeptidases are enzymes that hydrolyze C-terminal peptide bonds. The carboxypeptidase family includes metallo-, serine, and cysteine carboxypeptidases. According to their substrate specificity, these enzymes are referred to as carboxypeptidase A (cleaving aliphatic residues) or carboxypeptidase B (cleaving basic amino residues). CPB2 is activated by thrombin and acts on carboxypeptidase B substrates. After thrombin activation, the mature protein downregulates fibrinolysis. CPB2 is synthesized by the liver and circulates in the plasma as a plasminogen-bound zymogen. When it is activated by proteolysis at residue Arg92 by the thrombin/thrombomodulin complex. CPB2 cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. CPB2 exhibits carboxypeptidase activity and activated CPB2 reduces fibrinolysis by removing the fibrin C-terminal residues that are important for the binding and activation of plasminogen.

References

Eaton DL. et al.,1991, J Biol Chem. 266 (32): 21833-8. Boffa MB. et al.,1999, Biochemistry. 38 (20): 6547-58. Liu T. et al., 2005, J. Proteome Res. 4: 2070-80. Valnickova Z. et al., 2006, Biochemistry. 45: 1525-35. Chen R. et al., 2009, J. Proteome Res. 8: 651-61.

NCBI and Uniprot Product Information

• NCBI GI #: 126273569
• NCBI GeneID: 1361
• NCBI Accession #: NP_001863.2
• NCBI GenBank Nucleotide #: NM_001872.3
• UniProt Accession #: Q96IY4; Q15114; Q5T9K1; Q5T9K2; Q9P2Y6; A8K464
• Molecular Weight: 48,424 Da
• NCBI Official Full Name: carboxypeptidase B2 preproprotein
• NCBI Official Synonym Full Names: carboxypeptidase B2 (plasma)
• NCBI Official Symbol: CPB2
• NCBI Official Synonym Symbols: CPU; PCPB; TAFI
• NCBI Protein Information: carboxypeptidase B2; carboxypeptidase R; carboxypeptidase B-like protein; thrombin-activable fibrinolysis inhibitor; thrombin-activatable fibrinolysis inhibitor; carboxypeptidase B2 (plasma, carboxypeptidase U)
• UniProt Protein Name: Carboxypeptidase B2
• Protein Family: Carboxypeptidase
• UniProt Gene Name: CPB2
• UniProt Synonym Gene Names: CPU; pCPB; TAFI
• UniProt Entry Name: CBPB2_HUMAN

NCBI Description

Carboxypeptidases are enzymes that hydrolyze C-terminal peptide bonds. The carboxypeptidase family includes metallo-, serine, and cysteine carboxypeptidases. According to their substrate specificity, these enzymes are referred to as carboxypeptidase A (cleaving aliphatic residues) or carboxypeptidase B (cleaving basic amino residues). The protein encoded by this gene is activated by trypsin and acts on carboxypeptidase B substrates. After thrombin activation, the mature protein downregulates fibrinolysis. Polymorphisms have been described for this gene and its promoter region. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Uniprot Description

CPB2: Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down- regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Protease; Secreted, signal peptide; EC 3.4.17.20

Chromosomal Location of Human Ortholog: 13q14.11

Cellular Component: extracellular space

Molecular Function: zinc ion binding; metallocarboxypeptidase activity

Biological Process: response to drug; fibrinolysis; negative regulation of fibrinolysis; response to heat; blood coagulation; proteolysis