TRIM72 Antibody | anti-TRIM72 antibody

Goat anti-TRIM72, Biotinylated Antibody

Cat. Num. AAA423747

• Gene Names: TRIM72; MG53
• Reactivity: Mouse; Expected from sequence similarity: Human, Rat, Dog, Cow
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: TRIM72; Goat anti-TRIM72; Biotinylated Antibody; Goat Anti-TRIM72 Antibody; tripartite motif-containing 72; anti-TRIM72 antibody

• Reactivity: Mouse; Expected from sequence similarity: Human, Rat, Dog, Cow
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Sequence Length: 477

• Applicable Applications for anti-TRIM72 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Pep-ELISA: antibody detection limit dilution 1:16000.; WB: Approx 52kDa band observed in Mouse Heart lysates. Recommended concentration: 0.01-0.03ug/ml.
• Immunogen Sequence: Peptide with sequence C-RPQALSTNLQ., from the internal region of the protein sequence according to NP_001008275.1.
• Peptide Sequence + C: C-RPQALSTNLQ.
• Epitope: Internal Region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Biotinylated (0.01ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)

NCBI and Uniprot Product Information

• NCBI GI #: 56605981
• NCBI GeneID: 493829
• NCBI Accession #: NP_001008275.1; NP_001073401.1
• NCBI GenBank Nucleotide #: NM_001008274.2
• Molecular Weight: 52.8kDa
• NCBI Official Full Name: tripartite motif-containing 72
• NCBI Official Synonym Full Names: tripartite motif containing 72
• NCBI Official Symbol: TRIM72
• NCBI Official Synonym Symbols: MG53
• NCBI Protein Information: tripartite motif-containing protein 72
• UniProt Protein Name: Tripartite motif-containing protein 72
• Protein Family: Tripartite motif-containing protein
• UniProt Gene Name: TRIM72
• UniProt Synonym Gene Names: Mg53

Uniprot Description

Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca2+-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles ().