Western Blot (WB)
(DHRS2 Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the DHRS2 antibody detected the DHRS2 protein (arrow).)
Rabbit anti-Human DHRS2 Polyclonal Antibody | anti-DHRS2 antibody
DHRS2, CT (DHRS2, Dehydrogenase/reductase SDR family member 2, Dicarbonyl reductase HEP27, Protein D) (FITC)
Gene Names
DHRS2; HEP27; SDR25C1
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
DHRS2; Polyclonal Antibody; CT (DHRS2; Dehydrogenase/reductase SDR family member 2; Dicarbonyl reductase HEP27; Protein D) (FITC); Protein D; anti-DHRS2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-DHRS2 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:50-100
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
DHRS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 205-235 amino acids from the C-terminal region of human DHRS2.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(DHRS2 Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the DHRS2 antibody detected the DHRS2 protein (arrow).)
Western Blot (WB)
(DHRS2 Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the DHRS2 antibody detected the DHRS2 protein (arrow).)
Immunohistochemistry (IHC)
(DHRS2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DHRS2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(DHRS2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DHRS2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-DHRS2 antibody
DHRS2 displays NADPH-dependent dicarbonyl reductase activity in vitro with 3,4-Hexanedione, 2,3-Heptanedione and 1-Phenyl-1,2-propanedione as substrates. DHRS2 do not reductase activity is displayed in vitro with steroids, retinoids and sugars as substrates. This protein may inhibit cell replication.
Product Categories/Family for anti-DHRS2 antibody
References
Monge, M., et al. Carcinogenesis 30(8):1288-1297(2009). Persson, B., et al. Chem. Biol. Interact. 178 (1-3), 94-98 (2009). Shafqat, N., et al. Cell. Mol. Life Sci. 63(10):1205-1213(2006).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,496 Da
NCBI Official Full Name
dehydrogenase/reductase SDR family member 2, mitochondrial isoform 1
NCBI Official Synonym Full Names
dehydrogenase/reductase (SDR family) member 2
NCBI Official Symbol
DHRS2
NCBI Official Synonym Symbols
HEP27; SDR25C1
NCBI Protein Information
dehydrogenase/reductase SDR family member 2, mitochondrial; dehydrogenase/reductase member 2; dicarbonyl reductase HEP27; protein D; short chain dehydrogenase/reductase family 25C, member 1; short-chain alcohol dehydrogenase family member
UniProt Protein Name
Dehydrogenase/reductase SDR family member 2
Protein Family
UniProt Gene Name
DHRS2
UniProt Entry Name
DHRS2_HUMAN
Uniprot Description
DHRS2: Displays NADPH-dependent dicarbonyl reductase activity in vitro with 3,4-Hexanedione, 2,3-Heptanedione and 1-Phenyl-1,2- propanedione as substrates. No reductase activity is displayed in vitro with steroids, retinoids and sugars as substrates. May inhibit cell replication. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 1.1.1.-; Oxidoreductase; EC 1.1.-.-
Chromosomal Location of Human Ortholog: 14q11.2
Cellular Component: mitochondrion; mitochondrial matrix; cytoplasm; nuclear envelope; nucleus
Molecular Function: carbonyl reductase (NADPH) activity
Biological Process: negative regulation of cell proliferation; response to toxin; C21-steroid hormone metabolic process; myeloid dendritic cell differentiation; negative regulation of apoptosis
Research Articles on DHRS2
Similar Products
Product Notes
The DHRS2 dhrs2 (Catalog #AAA6292006) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DHRS2, CT (DHRS2, Dehydrogenase/reductase SDR family member 2, Dicarbonyl reductase HEP27, Protein D) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DHRS2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:50-100 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the DHRS2 dhrs2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DHRS2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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