Western Blot (WB)
((0. 01ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat TRIM72 Polyclonal Antibody | anti-TRIM72 antibody
Goat anti-TRIM72 Antibody
Immunohistochemistry: In paraffin embedded Human Skeletal Muscle shows staining of the fibre membranes, detached artificially by the sectioning. Recommended concentration, 3-5ug/ml.
Western Blot: Approx 52kDa band observed in Mouse and Rat Heart lysates (calculated MW of 52.8kDa according to Mouse NP_001073401.1). Recommended concentration: 0.01-0.03ug/ml.
Western Blot (WB)
((0. 01ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 01ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
Uniprot Description
TRIM72: Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles. Belongs to the TRIM/RBCC family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin conjugating system; Ubiquitin ligase
Chromosomal Location of Human Ortholog: 16p11.2
Cellular Component: cytoplasmic vesicle membrane; sarcolemma
Molecular Function: phosphatidylserine binding; protein binding; ubiquitin conjugating enzyme binding; zinc ion binding
Biological Process: exocytosis; muscle contraction; muscle development; muscle system process; negative regulation of insulin receptor signaling pathway; negative regulation of insulin-like growth factor receptor signaling pathway; plasma membrane repair; proteasomal ubiquitin-dependent protein catabolic process; protein homooligomerization; protein ubiquitination
Research Articles on TRIM72
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Product Notes
The TRIM72 trim72 (Catalog #AAA422489) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-TRIM72 Antibody reacts with Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's TRIM72 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 16000. Immunohistochemistry: In paraffin embedded Human Skeletal Muscle shows staining of the fibre membranes, detached artificially by the sectioning. Recommended concentration, 3-5ug/ml. Western Blot: Approx 52kDa band observed in Mouse and Rat Heart lysates (calculated MW of 52.8kDa according to Mouse NP_001073401.1). Recommended concentration: 0.01-0.03ug/ml. Researchers should empirically determine the suitability of the TRIM72 trim72 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RPQALSTNLQ. It is sometimes possible for the material contained within the vial of "TRIM72, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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