Western Blot (WB)
(DMRTA2 Antibody (C-term) western blot analysis in PC-3 cell line, mouse testis and zebra fish brain tissue lysates (35ug/lane).This demonstrates the DMRTA2 antibody detected the DMRTA2 protein (arrow).)
Rabbit anti-Human DMRTA2 Polyclonal Antibody | anti-DMRTA2 antibody
DMRTA2, CT (DMRTA2, DMRT5, Doublesex-and mab-3-related transcription factor A2, Doublesex-and mab-3-related transcription factor 5) (PE)
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
DMRTA2; Polyclonal Antibody; CT (DMRTA2; DMRT5; Doublesex-and mab-3-related transcription factor A2; Doublesex-and mab-3-related transcription factor 5) (PE); Doublesex-and mab-3-related transcription factor 5; anti-DMRTA2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-DMRTA2 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
DMRTA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 487-515 amino acids from the C-terminal region of human DMRTA2.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(DMRTA2 Antibody (C-term) western blot analysis in PC-3 cell line, mouse testis and zebra fish brain tissue lysates (35ug/lane).This demonstrates the DMRTA2 antibody detected the DMRTA2 protein (arrow).)
Western Blot (WB)
(DMRTA2 Antibody (C-term) western blot analysis in PC-3 cell line, mouse testis and zebra fish brain tissue lysates (35ug/lane).This demonstrates the DMRTA2 antibody detected the DMRTA2 protein (arrow).)
Immunofluorescence (IF)
(Fluorescent confocal image of U251 cell stained with DMRTA2 Antibody (C-term).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with DMRTA2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). DMRTA2 immunoreactivity is localized to Microtubules significantly.)
Immunofluorescence (IF)
(Fluorescent confocal image of U251 cell stained with DMRTA2 Antibody (C-term).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with DMRTA2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). DMRTA2 immunoreactivity is localized to Microtubules significantly.)
Product Categories/Family for anti-DMRTA2 antibody
References
Ottolenghi, C., et al. Genomics 79(3):333-343(2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53,356 Da
NCBI Official Full Name
doublesex- and mab-3-related transcription factor A2
NCBI Official Synonym Full Names
DMRT-like family A2
NCBI Official Symbol
DMRTA2
NCBI Protein Information
doublesex- and mab-3-related transcription factor A2; OTTHUMP00000212959; doublesex- and mab-3-related transcription factor 5
UniProt Protein Name
Doublesex- and mab-3-related transcription factor A2
Protein Family
UniProt Gene Name
DMRTA2
UniProt Synonym Gene Names
DMRT5
UniProt Entry Name
DMTA2_HUMAN
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Product Notes
The DMRTA2 dmrta2 (Catalog #AAA6292508) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DMRTA2, CT (DMRTA2, DMRT5, Doublesex-and mab-3-related transcription factor A2, Doublesex-and mab-3-related transcription factor 5) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DMRTA2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the DMRTA2 dmrta2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DMRTA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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