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SDS-Page (Recombinant SARS-CoV-2 Spike protein, S1 (aa319-541) protein gel. 1 ug of SARS-CoV-2 Spike protein was run on a 4-12% SDS-PAGE gel, stained with Coomassie blue. MW: 40 kDa Purity: >90%)

COVID 19 Spike, S1 RBD Coronavirus Recombinant Protein | COVID-19 recombinant protein

Recombinant SARS-CoV-2 Spike protein, S1 (RBD aa319-541)

Purity
The recombinant protein is >90% pure by SDS-PAGE.
Synonyms
COVID 19 Spike; S1 RBD Coronavirus; Recombinant SARS-CoV-2 Spike protein; S1 (RBD aa319-541); 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike; S1 RBD; COVID-19 recombinant protein
Ordering
For Research Use Only!
Host
293 cells
Purity/Purification
The recombinant protein is >90% pure by SDS-PAGE.
Form/Format
Recombinant SARS-CoV-2 Spike protein is supplied in 25mM Tris-HCl, pH8.0, 270mM NaCl, and 10% glycerol.
Protein Species
Virus
Tag
His-Tag
Notes
This product was manufactured as described in Protein Details and has been validated for use in ELISA. All available data for the protein is shown.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice

SDS-Page

(Recombinant SARS-CoV-2 Spike protein, S1 (aa319-541) protein gel. 1 ug of SARS-CoV-2 Spike protein was run on a 4-12% SDS-PAGE gel, stained with Coomassie blue. MW: 40 kDa Purity: >90%)

SDS-Page (Recombinant SARS-CoV-2 Spike protein, S1 (aa319-541) protein gel. 1 ug of SARS-CoV-2 Spike protein was run on a 4-12% SDS-PAGE gel, stained with Coomassie blue. MW: 40 kDa Purity: >90%)

Testing Data

(SARS-CoV-2 Spike protein, S1 RBD Competition and Blocking Activity Assay A) SARS-CoV-2 Spike Antibody (clone AM001414) competes with ACE2 receptor for RBD binding. SARS-CoV-2 Spike Antibody (clone AM001414) was serially diluted in the presence of 100 ng/mL biotinylated ACE2 receptor, then added to wells coated with 1 ug/mL RBD, aa319-541 for 30 min. Streptavidin-HRP was then added to detect bound ACE2 receptor. B) SARS-CoV-2 Spike Antibody (clone AM001414) blocks ACE2 receptor from binding to RBD. Serial dilutions of SARS-CoV-2 Spike Antibody (clone AM001414) was added to wells coated with 1 ug/mL RBD, aa319-541. 100 ng/mL biotinylated ACE2 receptor was then added for 30 min, followed by Streptavidin-HRP to detect bound ACE2 receptor.)

Testing Data (SARS-CoV-2 Spike protein, S1 RBD Competition and Blocking Activity Assay A) SARS-CoV-2 Spike Antibody (clone AM001414) competes with ACE2 receptor for RBD binding. SARS-CoV-2 Spike Antibody (clone AM001414) was serially diluted in the presence of 100 ng/mL biotinylated ACE2 receptor, then added to wells coated with 1 ug/mL RBD, aa319-541 for 30 min. Streptavidin-HRP was then added to detect bound ACE2 receptor. B) SARS-CoV-2 Spike Antibody (clone AM001414) blocks ACE2 receptor from binding to RBD. Serial dilutions of SARS-CoV-2 Spike Antibody (clone AM001414) was added to wells coated with 1 ug/mL RBD, aa319-541. 100 ng/mL biotinylated ACE2 receptor was then added for 30 min, followed by Streptavidin-HRP to detect bound ACE2 receptor.)
Related Product Information for COVID-19 recombinant protein
Short Description: Recombinant SARS-CoV-2 Spike protein was expressed in 293 cells and contains a C-terminal 6XHis-Tag with an observed molecular weight of 40 kDa. The recombinant protein is >90% pure by SDS-PAGE. This protein has been validated for use in ELISA.

Background: COVID-19, which is short for coronavirus disease 2019, is the official name of the respiratory disease caused by infection with the novel coronavirus SARS-CoV-2. The virus that causes COVID-19 was named SARS-CoV-2 because it is a coronavirus genetically similar to, yet distinct from, the virus that caused the severe acute respiratory syndrome (SARS) outbreak in 2003. Studying the details of how this virus replicates and causes the disease will allow scientists and physicians to more rapidly develop fast and accurate methods of detection as well as to deploy therapeutic and vaccine strategies.
Product Categories/Family for COVID-19 recombinant protein

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Product Notes

The COVID-19 (Catalog #AAA389387) is a Recombinant Protein produced from 293 cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "COVID 19 Spike, S1 RBD Coronavirus, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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