Human Kallikrein 8 (KLK8) ELISA Kit | KLK8 elisa kit

Human Kallikrein 8 (KLK8) ELISA Kit

Cat. Num. AAA263112

• Gene Names: KLK8; NP; HNP; NRPN; PRSS19; TADG14
• Reactivity: Human
• Synonyms: Kallikrein 8 (KLK8); Human Kallikrein 8 (KLK8) ELISA Kit; KLK8 elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.
• Sequence Length: 139

• Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant KLK8 concentration
• Assay Type: Sandwich
• Detection Range: 10 ng/ml-0.156 ng/ml
• Sensitivity: 0.05 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KLK8 elisa kit

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human KLK8 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 527121930
• NCBI GeneID: 11202
• NCBI Accession #: NP_001268360.1
• NCBI GenBank Nucleotide #: NM_001281431.1
• Molecular Weight: 3,653 Da
• NCBI Official Full Name: kallikrein-8 isoform 5
• NCBI Official Synonym Full Names: kallikrein-related peptidase 8
• NCBI Official Symbol: KLK8
• NCBI Official Synonym Symbols: NP; HNP; NRPN; PRSS19; TADG14
• NCBI Protein Information: kallikrein-8; ovasin; serine protease 19; serine protease TADG-14; tumor-associated differentially expressed gene 14 protein
• UniProt Protein Name: Kallikrein-8
• Protein Family: Kallikrein
• UniProt Gene Name: KLK8
• UniProt Synonym Gene Names: NRPN; PRSS19; TADG14; UNQ283/PRO322; hK8; NP
• UniProt Entry Name: KLK8_HUMAN

NCBI Description

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in tandem in a gene cluster on chromosome 19. The encoded protein may be involved in proteolytic cascade in the skin and may serve as a biomarker for ovarian cancer. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

Uniprot Description

KLK8: Serine protease which is capable of degrading a number of proteins such as casein, fibrinogen, kininogen, fibronectin and collagen type IV. Also cleaves L1CAM in response to increased neural activity. Induces neurite outgrowth and fasciculation of cultured hippocampal neurons. Plays a role in the formation and maturation of orphan and small synaptic boutons in the Schaffer- collateral pathway, regulates Schaffer-collateral long-term potentiation in the hippocampus and is required for memory acquisition and synaptic plasticity. Involved in skin desquamation and keratinocyte proliferation. Plays a role in the secondary phase of pathogenesis following spinal cord injury. Belongs to the peptidase S1 family. Kallikrein subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.21.118; Cell development/differentiation; Secreted; Protease; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: extracellular space; cytoplasm

Molecular Function: protein binding; serine-type endopeptidase activity

Biological Process: cell death; negative regulation of myelination; negative regulation of axon regeneration; response to wounding; synapse organization and biogenesis; regulation of synapse organization and biogenesis; keratinocyte proliferation; proteolysis; memory; neurite morphogenesis