ELISA (EIA)
(SARS-CoV-2 Spike Antibody (clone AM009105) tested by ELISA. SARS-CoV-2 Spike RBD protein was coated onto microtiter plates at 0.5 ug/mL and then incubated with a dilution series of SARS-CoV-2 Spike Antibody (clone AM009105). Bound antibodies were detected with anti-human IgG conjugated to horseradish peroxidase (HRP) followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm. )
Human COVID 19 Spike Coronavirus Antibody | anti-COVID-19 antibody
SARS-CoV-2 Spike Antibody (AM009105)
Reactivity
Virus
Applications
ELISA
Purity
Protein A Chromatography
Synonyms
COVID 19 Spike Coronavirus; SARS-CoV-2 Spike Antibody (AM009105); 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike; anti-COVID-19 antibody
Host
Human
Reactivity
Virus
Isotype
IgG1, k
Clone Number
AM009105
Purity/Purification
Protein A Chromatography
Form/Format
140mM Hepes, pH7.5, 70mM NaCl, 32mM NaOAc, 0.035% sodium azide, and 30% glycerol. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-COVID-19 antibody
ELISA (EIA)
Application Notes
Applications Validated:
ELISA
This antibody has been tested by ELISA and is specific for SARS-CoV-2 Spike protein S1 Subunit of Receptor Binding Domain (RBD).
Note: Clone AM009105, developed, was originally published as Clone 105-9. Details on the development and characterization of this clone are available here: Wan J., et. al. Human IgG neutralizing monoclonal antibodies block SARS-CoV-2 infection. Cell Reports, 32: 107918, July 21, 2020.
ELISA
This antibody has been tested by ELISA and is specific for SARS-CoV-2 Spike protein S1 Subunit of Receptor Binding Domain (RBD).
Note: Clone AM009105, developed, was originally published as Clone 105-9. Details on the development and characterization of this clone are available here: Wan J., et. al. Human IgG neutralizing monoclonal antibodies block SARS-CoV-2 infection. Cell Reports, 32: 107918, July 21, 2020.
Antibody Type
Recombinant Antibody
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
ELISA (EIA)
(SARS-CoV-2 Spike Antibody (clone AM009105) tested by ELISA. SARS-CoV-2 Spike RBD protein was coated onto microtiter plates at 0.5 ug/mL and then incubated with a dilution series of SARS-CoV-2 Spike Antibody (clone AM009105). Bound antibodies were detected with anti-human IgG conjugated to horseradish peroxidase (HRP) followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm. )
ELISA (EIA)
(SARS-CoV-2 Spike Antibody (clone AM009105) tested by ELISA. SARS-CoV-2 Spike RBD protein was coated onto microtiter plates at 0.5 ug/mL and then incubated with a dilution series of SARS-CoV-2 Spike Antibody (clone AM009105). Bound antibodies were detected with anti-human IgG conjugated to horseradish peroxidase (HRP) followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm. )
Related Product Information for anti-COVID-19 antibody
Short Description: Our SARS-CoV-2 Spike Antibody (AM009105) was raised in a Human host. It has been validated for use in ELISA; it has been shown to react with Virus samples.
Background: COVID-19, which is short for coronavirus disease 2019, is the official name of the respiratory disease caused by infection with the novel coronavirus SARS-CoV-2. The virus that causes COVID-19 was named SARS-CoV-2 because it is a coronavirus genetically similar to, yet distinct from, the virus that caused the severe acute respiratory syndrome (SARS) outbreak in 2003. Studying the details of how this virus replicates and causes the disease will allow scientists and physicians to more rapidly develop fast and accurate methods of detection as well as to deploy therapeutic and vaccine strategies. This antibody was derived from COVID-19 patients who have cleared the virus. Patient serum IgG was sequenced and expressed as full-length IgG1 with human immunoglobulin heavy and light chains in mammalian 293 cells.
Background: COVID-19, which is short for coronavirus disease 2019, is the official name of the respiratory disease caused by infection with the novel coronavirus SARS-CoV-2. The virus that causes COVID-19 was named SARS-CoV-2 because it is a coronavirus genetically similar to, yet distinct from, the virus that caused the severe acute respiratory syndrome (SARS) outbreak in 2003. Studying the details of how this virus replicates and causes the disease will allow scientists and physicians to more rapidly develop fast and accurate methods of detection as well as to deploy therapeutic and vaccine strategies. This antibody was derived from COVID-19 patients who have cleared the virus. Patient serum IgG was sequenced and expressed as full-length IgG1 with human immunoglobulin heavy and light chains in mammalian 293 cells.
Product Categories/Family for anti-COVID-19 antibody
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
Mol_Mass: 47.9 kDa; AP_Mol_Mass: 50-60 kDa
Similar Products
Product Notes
The COVID-19 (Catalog #AAA389523) is an Antibody produced from Human and is intended for research purposes only. The product is available for immediate purchase. The SARS-CoV-2 Spike Antibody (AM009105) reacts with Virus and may cross-react with other species as described in the data sheet. AAA Biotech's COVID 19 Spike Coronavirus can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Applications Validated: ELISA This antibody has been tested by ELISA and is specific for SARS-CoV-2 Spike protein S1 Subunit of Receptor Binding Domain (RBD). Note: Clone AM009105, developed, was originally published as Clone 105-9. Details on the development and characterization of this clone are available here: Wan J., et. al. Human IgG neutralizing monoclonal antibodies block SARS-CoV-2 infection. Cell Reports, 32: 107918, July 21, 2020. Researchers should empirically determine the suitability of the COVID-19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COVID 19 Spike Coronavirus, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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