Human COVID 19 Spike Coronavirus Antibody | anti-COVID-19 antibody
SARS-CoV-2 Spike Antibody (AM020553)(Clone AM020553 (553-20))
Reactivity
Virus
Applications
ELISA
Purity
Protein A Chromatography
Synonyms
COVID 19 Spike Coronavirus; SARS-CoV-2 Spike Antibody (AM020553)(Clone AM020553 (553-20)); 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike; anti-COVID-19 antibody
Host
Human
Reactivity
Virus
Isotype
IgG1
Clone Number
AM020553 (553-20)
Purity/Purification
Protein A Chromatography
Form/Format
140mM Hepes, pH7.5, 70mM NaCl, 32mM NaOAc, 0.035% sodium azide, and 30% glycerol. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-COVID-19 antibody
ELISA (EIA)
Application Notes
Applications Validated:
ELISA: 0.01-1ug/ml
Note: Clone AM020553, developed, was originally published as Clone 553-20. Details on the development and characterization of this clone are available here: Wan J., et. al. Human IgG neutralizing monoclonal antibodies block SARS-CoV-2 infection. Cell Reports, 32: 107918, July 21, 2020.
ELISA: 0.01-1ug/ml
Note: Clone AM020553, developed, was originally published as Clone 553-20. Details on the development and characterization of this clone are available here: Wan J., et. al. Human IgG neutralizing monoclonal antibodies block SARS-CoV-2 infection. Cell Reports, 32: 107918, July 21, 2020.
Antibody Type
Recombinant Antibody
Immunogen
N/A-derived from COVID-19 patients who have cleared the virus. Their antibodies were screened for reactivity to SARS-CoV-2 and then sequenced and expressed as recombinant antibodies.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
ELISA (EIA)
(AbFlex SARS-CoV-2 Spike Antibody (rAb) (AM020553) tested by ELISA using SARS Spike protein ECD. SARS-CoV-2 Spike Extracellular Domain (ECD a.a. 14-1213) protein was coated onto microtiter plates at 5 ug/mL and then incubated with a dilution series of SARS-CoV-2 Spike Antibody (rAb) (clone AM020553). Bound antibodies were detected with anti-human IgG conjugated to horseradish peroxidase (HRP) followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.)
Related Product Information for anti-COVID-19 antibody
Short Description: Our SARS-CoV-2 Spike Antibody (AM020553)(Clone AM020553 (553-20)) was raised in a Human host. It has been validated for use in ELISA; it has been shown to react with Virus samples.
Background: COVID-19, which is short for coronavirus disease 2019, is the official name of the respiratory disease caused by infection with the novel coronavirus SARS-CoV-2. The virus that causes COVID-19 was named SARS-CoV-2 because it is a coronavirus genetically similar to, yet distinct from, the virus that caused the severe acute respiratory syndrome (SARS) outbreak in 2003. Studying the details of how this virus replicates and causes the disease will allow scientists and physicians to more rapidly develop fast and accurate methods of detection as well as to deploy therapeutic and vaccine strategies. This antibody was derived from COVID-19 patients who have cleared the virus. Patient serum IgG was sequenced and expressed as full-length IgG1 with human immunoglobulin heavy and light chains in mammalian 293 cells.
Background: COVID-19, which is short for coronavirus disease 2019, is the official name of the respiratory disease caused by infection with the novel coronavirus SARS-CoV-2. The virus that causes COVID-19 was named SARS-CoV-2 because it is a coronavirus genetically similar to, yet distinct from, the virus that caused the severe acute respiratory syndrome (SARS) outbreak in 2003. Studying the details of how this virus replicates and causes the disease will allow scientists and physicians to more rapidly develop fast and accurate methods of detection as well as to deploy therapeutic and vaccine strategies. This antibody was derived from COVID-19 patients who have cleared the virus. Patient serum IgG was sequenced and expressed as full-length IgG1 with human immunoglobulin heavy and light chains in mammalian 293 cells.
Product Categories/Family for anti-COVID-19 antibody
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
Mol_Mass: 47.9 kDa; AP_Mol_Mass: 50-60 kDa
