CD279 protein

CD279-muIg-Purified Preservative Free

Cat. Num. AAA666786

• Reactivity: Human
• Applications: ELISA, Flow Cytometry, Functional Assay
• Purity: Purified Preservative Free
• Synonyms: CD279; CD279-muIg-Purified Preservative Free; Human CD279(PD-1)-muIg Fusion Protein; CD279 protein

• Host: CHO cells
• Reactivity: Human
• Purity/Purification: Purified Preservative Free
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl
• Concentration: 0.5 mg/ml (varies by lot)

• Applicable Applications for CD279 protein: ELISA (EIA), Flow Cytometry (FC/FACS)
• Molecular Structure: A soluble fusion protein consisting of the extracellular (139aa) domain of human CD279(PD-1) and murine IgG2a Fc +hinge. In SDS-PAGE, this protein runs at 52 kd under native, and 27 kd under reducing conditions. Data suggest that the murine Ig Fc portion of this construct may be missing.
• Transfectant Cell Line: CHO
• Performance: Five x 105 cultured human monocyte-derived Dendritic cells (moDC) were washed and incubated 45 minutes on ice with 80 ul of CD279-muIg/Biotin at 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Streptavidin/PE, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.57 log10 fluorescent units. This binding was blocked when cells were pre incubated with 100 ug/ml of purified CD279-muIg.
• Production: CD279-muIg was purified from (low FBS containing) tissue culture supernatant of CHO transfectants using Protein A and size exclusion chromatography. Purity was >95% by SDS-PAGE.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl. Product was 0.1 um filtered and vialed under aseptic conditions.
• Preparation and Storage: Store at 2 - 5 degree C. Freeze/Thawing is not recommended.; Product should retain activity for at least 6 months after shipping date when stored as recommended.

Testing Data

Related Product Information for CD279 protein

Information: Human CD279 (PD-1, Programmed death 1) is a 55 kd Ig superfamily member with similarity to CD28 and CD152(CTLA-4). It is expressed on activated T and B and myeloid cells, and engagement by its ligands PD-L1 (CD274, B7-H1) or PD-L2 (B7-DC) can inhibit proliferation and cytokine expression. In mice blockade of PD-1 ligand interaction has been used to augment T cell anti cancer responses (1,2,3). Recombinant CD279-muIg binds to recombinant CD274 and CD273 in EIA and cell surface CD273 on moDC in FACS. It is recognized by anti-CD279 mAb in EIA.

References

1) MA Curran, Allison JP, et al. (2010) PNAS 107(9): 4275-80. 2) Mangsbo SM, TH Totterman, et al. (2010) J Immunotherapy 33(3):225. 3) Hirano F, L Chen, et al. (2005) Canc Res 65: 1089.