CD275 protein

CD275-muIg-Biotin

Cat. Num. AAA666853

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA
• Synonyms: CD275; CD275-muIg-Biotin; Human CD275(ICOSL; GL50; B7-H2)trn-muIg/Biotin; CD275 protein

• Host: CHO cells
• Reactivity: Human
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
• Concentration: 0.5 mg/ml (varies by lot)

• Applicable Applications for CD275 protein: Flow Cytometry (FC/FACS), ELISA (EIA)
• Molecular Structure: A soluble molecule consisting of the mature extracellular (213 aa) domain of human CD275 (GL50,ICOS L) fused to the murine IgG2a Fc (233 aa). (449 aa total). The molecule is dimeric with a predicted monomeric non glycosylated molecular weight of 50.3 kd.
• Transfectant Cell Line: CHO
• Performance: Five x 105 human HPB-MLT cells per tube were washed and incubated 45 minutes on ice with 80 mul of CD275trn-muIg/Biotin at a concentration of 5 mug/ml. Cells were washed twice and incubated with 2 degree reagent Streptavidin/R-PE, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.44 log10 fluorescent units when compared to a Mouse IgG2a/Biotin negative control at a similar concentration.
• Conjugation: Biotin
• Production: Fusion protein from (low FBS containing) tissue culture supernatant of transfectants was purified using affinity and size exclusion chromatography), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate by diafiltration.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
• Preparation and Storage: Store at 2 - 5 degree C. Freeze/Thawing is not recommended.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for CD275 protein

Information: The inducible costimulator (ICOS, T cell activation molecule H4) is similar to human CD28 (24% homology), and plays an analogous role in the T cell activation process. Unlike CD28, ICOS is only expressed on activated T cells. Secondary signaling through CD28 or ICOS results in discrete cytokine secretion profiles by the activated T cells(1). Engagement of CD152 (CTLA-4) anergizes cells costimulated with either CD28 and ICOS(2). Signaling through ICOS is particularly important in progression of TH2 immune response (5). The receptor for human ICOS is GL50(ICOS L), a member of the B7 family sharing ~20% homology with CD80 (B7-1) and CD86 (B7-2)(3). Two RNA splice variants exist for this molecule, differing only in the cytoplasmic domain(4). Blockade of the ICOS-GL50 interaction in mice improves allograft survival(6) and reduces EAE(7). Recombinant human CD275trn-muIg binds to recombinant and native CD278(ICOS) in EIA and Flow cytometry. Its binding is blocked by pre incubation with soluble recombinant CD278-muIg.

References

1) Beier, K.C., R.A. Kroczek, et al. 2000, Eur J Immunol. 30(12):3707-3717. 2) Riley, J.L., C.H. June, et al. 2001, J. Immunol. 166: 4943-4948. 3) Ling, V., M. Collins, et al. 2000, J. Immunol. 164: 1653-1657. 4) Ling, V., M. Collins, et al. 2001, J. Immunol. 166: 7300-7308. 5) K.C. Beier, et al, (2000) Eur J Immunol 30: 3707-3717. 6) E. Ozkaynak, et al, (2001) Nat Immunol 2: 591-596. 7) J.B. Rottman, et al, (2001) Nat Immunol 2: 605-611.