Mouse anti-Human CD279 Monoclonal Antibody | anti-CD279 antibody

anti-human CD279(PD-1)-FITC

Cat. Num. AAA666604

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA
• Synonyms: CD279; Monoclonal Antibody; anti-human CD279(PD-1)-FITC; Monoclonal anti-human CD279(PD-1)/Biotin; anti-CD279 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1k
• Clone Number: ANC4H6
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
• Concentration: 1.0 mg/ml (varies by lot)

• Applicable Applications for anti-CD279 antibody: Flow Cytometry (FC/FACS), ELISA (EIA)
• Performance: Ficoll prepared human peripheral blood mononuclear cells (PBMC) were stimulated 5 days in culture with 5 ug/ml Phytohemagglutanin (PHA). Five x 105 cells per tube were pre incubated 10 minutes with 250 ug/ml human IgG (to block nonspecific binding) after which they were incubated 45 minutes on ice with 80 ul of product at a concentration of 10 ug/ml. Cells were then washed twice and incubated 45 minutes with 20 ul of 2 degree reagent Streptavidin/R-PE, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.04 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was blocked when reagent was pre incubated 10 minutes with a ten-fold excess of recombinant CD279-muIg.
• Conjugation: FITC
• Production: Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to 95% Immunoglobulin by SDS-PAGE, and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
• Immunogen: Recombinant human 279
• Preparation and Storage: Store at 2 - 5 degree C. Freeze/thawing not recommended.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for anti-CD279 antibody

Information: Human CD279 (PD-1, Programmed death 1) is a 55 kd Ig superfamily member with sililarity to CD28 and CD152(CTLA-4). It is expressed on activated T and B and myeloid cells, and engagement by its ligands PD-L1 (CD274, B7-H1) or PD-L2 (B7-DC) can inhibit proliferation and cytokine expression. In mice blockade of PD-1 ligand interaction has been used to augment T cell anti cancer responses (3,4,5). Anti-CD279 antibody clone ANC4H6 binds to CD279 present on PHA activated PBMC in FACS, and binds to recombinant CD279 in EIA.

References

1) Ishida et al. (1992) EMBO J 11(11):3887-95. 2) Carreno BM, M Collins, et al. (2001) Annu Rev Immunol 20:29. 3) MA Curran, Allison JP, et al. (2010) PNAS 107(9): 4275-80. 4) Mangsbo SM, TH Totterman, et al. (2010) J Immunotherapy 33(3):225. 5) Hirano F, L Chen, et al. (2005) Canc Res 65: 1089.