5'-Nucleotidase Active Protein | Nt5e active protein

5'-Nucleotidase, Recombinant, Human (CD73, 5'-nucleotidase, ecto)

Cat. Num. AAA635626

• Gene Names: Nt5e; Nt5; CD73
• Purity: Purified; 90% by SDS­PAGE under reducing conditions and visulized by silver stain. Endotoxin: 1EU/ug (LAL)
• Synonyms: 5'-Nucleotidase; Recombinant; Human (CD73; 5'-nucleotidase; ecto); Nt5e active protein

• Host: CHO cells
• Purity/Purification: Purified; 90% by SDS­PAGE under reducing conditions and visulized by silver stain. Endotoxin: 1EU/ug (LAL)
• Form/Format: Supplied as a liquid in 20mM Tris, pH 7.5, 120mM sodium chloride, 4mM calcium chloride, 20% glycerol.

• Application Notes: SDS-PAGE: 61-62kD under reducing conditions
• Biological Activity: Measured by its ability to hydrolyze the 5'-phosphate group from the substrate adenosine-5'-monophosphate. The specific activity is 15,000pmoles/min/ug.
• Preparation and Storage: For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for Nt5e active protein

CD73, an ecto-5'-Nucleotidase, is an ectoenzyme expressed by most cell types. The 5'-Nucleotidase activity of CD73 converts extracellular nucleoside-5'-monophosphates to nucleosides, with AMP as the preferred substrate. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. These functions include the adhesion of B-cells to follicular dendritic cells and T-cell signaling CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'-Nucleotidase activity. The native enzyme is a homodimer bound to the cell membrane through a glycosyl phophatidylinositol (GPI) anchor. This recombinant human CD73 is truncated at the C-terminus, and lacks the GPI modification site. The recombinant CD73 is therefore secreted as a monomer.

Recombinant protein corresponding to Trp27-Lys547, with a C-terminal 6-His tag from human 5'-Nucleotidase expressed in CHO cells (AAH65937).

Product Categories/Family for Nt5e active protein

Molecular Biology; MB-Enzymes; Nucleotidase

NCBI and Uniprot Product Information

• NCBI GI #: 148747417
• NCBI GeneID: 58813
• NCBI Accession #: NP_067587.2
• NCBI GenBank Nucleotide #: NM_021576.2
• UniProt Accession #: P21588
• Molecular Weight: 59kD
• NCBI Official Full Name: 5'-nucleotidase
• NCBI Official Synonym Full Names: 5' nucleotidase, ecto
• NCBI Official Symbol: Nt5e
• NCBI Official Synonym Symbols: Nt5; CD73
• NCBI Protein Information: 5'-nucleotidase; 5'-NT; ecto-5'-nucleotidase
• UniProt Protein Name: 5'-nucleotidase
• Protein Family: 5'-nucleotidase
• UniProt Gene Name: Nt5e
• UniProt Synonym Gene Names: Nt5; Nte; 5'-NT
• UniProt Entry Name: 5NTD_RAT

NCBI Description

may hydrolyze extracellular nucleotides into membrane permeable nucleosides [RGD, Feb 2006]

Uniprot Description

NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Phosphatase (non-protein); Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; EC 3.1.3.5; Membrane protein, GPI anchor; Membrane protein, integral; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide

Cellular Component: cell surface; membrane; cytoplasm; integral to membrane; plasma membrane

Molecular Function: 5'-nucleotidase activity; ferrous iron binding; metal ion binding; nucleotide binding

Biological Process: dephosphorylation; negative regulation of inflammatory response; adenosine biosynthetic process; purine nucleotide biosynthetic process; brain development; positive regulation of lipid biosynthetic process; adenosine metabolic process; AMP catabolic process; response to aluminum ion