Rat 5'-nucleotidase ELISA Kit | Nt5e elisa kit

Rat 5'-nucleotidase ELISA Kit

Cat. Num. AAA762767

• Gene Names: Nt5e; Nt5; CD73
• Reactivity: Rat
• Synonyms: 5'-nucleotidase; Rat 5'-nucleotidase ELISA Kit; Nt5e; Nt5e elisa kit

• Reactivity: Rat
• Sequence Length: 576

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 3.12-200pg/ml
• Sensitivity: 1.875pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Nt5e elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Nt5e antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Nt5e antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Nt5e amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat Nt5e can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 148747417
• NCBI GeneID: 58813
• NCBI Accession #: NP_067587.2
• NCBI GenBank Nucleotide #: NM_021576.2
• Molecular Weight: 63,969 Da
• NCBI Official Full Name: 5'-nucleotidase
• NCBI Official Synonym Full Names: 5' nucleotidase, ecto
• NCBI Official Symbol: Nt5e
• NCBI Official Synonym Symbols: Nt5; CD73
• NCBI Protein Information: 5'-nucleotidase
• UniProt Protein Name: 5'-nucleotidase
• Protein Family: 5'-nucleotidase
• UniProt Gene Name: Nt5e
• UniProt Synonym Gene Names: Nt5; Nte; 5'-NT
• UniProt Entry Name: 5NTD_RAT

NCBI Description

may hydrolyze extracellular nucleotides into membrane permeable nucleosides [RGD, Feb 2006]

Uniprot Description

NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleotide Metabolism - purine; EC 3.1.3.5; Membrane protein, integral; Phosphatase (non-protein); Nucleotide Metabolism - pyrimidine; Membrane protein, GPI anchor; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide

Cellular Component: cell surface; cytoplasm; integral to membrane; membrane; plasma membrane

Molecular Function: 5'-nucleotidase activity; ferrous iron binding; metal ion binding; nucleotide binding

Biological Process: adenosine biosynthetic process; adenosine metabolic process; AMP catabolic process; brain development; dephosphorylation; leukocyte adhesion; negative regulation of inflammatory response; positive regulation of lipid biosynthetic process; purine nucleotide biosynthetic process; response to aluminum ion