Testing Data
(Human Placenta (FFPE) after heat-induced antigen retrieval using MBS6006096 (10ug/ml).)
Rabbit anti-Human 5'-Nucleotidase Polyclonal Antibody | anti-Nt5e antibody
5'-Nucleotidase, CT (CD73, CD73 Antigen, Ecto-5'nucleotidase, 5'-NT, eN, eNT, NT5, NT5E, NTE, Purine 5 Prime Nucleotidase)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Dilution: ELISA: 1:1000
Western Blot: 1:100-1:500
Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
Testing Data
(Human Placenta (FFPE) after heat-induced antigen retrieval using MBS6006096 (10ug/ml).)
Testing Data
(Human Placenta (FFPE) after heat-induced antigen retrieval using MBS6006096 (10ug/ml).)
Western Blot (WB)
(Western Blot analysis of mouse brain tissue lysate using MBS6006096.)
Western Blot (WB)
(Western Blot analysis of mouse brain tissue lysate using MBS6006096.)
NCBI and Uniprot Product Information
NCBI Description
may hydrolyze extracellular nucleotides into membrane permeable nucleosides [RGD, Feb 2006]
Uniprot Description
NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Phosphatase (non-protein); Membrane protein, integral; Nucleotide Metabolism - pyrimidine; Membrane protein, GPI anchor; EC 3.1.3.5; Nucleotide Metabolism - purine
Cellular Component: cell surface; membrane; cytoplasm; plasma membrane; integral to membrane
Molecular Function: 5'-nucleotidase activity; metal ion binding; ferrous iron binding; nucleotide binding
Biological Process: dephosphorylation; negative regulation of inflammatory response; adenosine biosynthetic process; positive regulation of lipid biosynthetic process; brain development; purine nucleotide biosynthetic process; adenosine metabolic process; AMP catabolic process; response to aluminum ion
Research Articles on Nt5e
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Product Notes
The Nt5e nt5e (Catalog #AAA6006096) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The 5'-Nucleotidase, CT (CD73, CD73 Antigen, Ecto-5'nucleotidase, 5'-NT, eN, eNT, NT5, NT5E, NTE, Purine 5 Prime Nucleotidase) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's 5'-Nucleotidase can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot, ELISA and Immunohistochemistry. Dilution: ELISA: 1:1000 Western Blot: 1:100-1:500 Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml. Researchers should empirically determine the suitability of the Nt5e nt5e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "5'-Nucleotidase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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