SDS-Page
5'-Nucleotidase/5'-NT/CD73 Active Protein | 5'-NT/CD73 active protein
Recombinant Human 5'-Nucleotidase/5'-NT/CD73 (AAH65937.1, C-Fc)
Purity
>95% as determined by reducing SDS-PAGE
Synonyms
5'-Nucleotidase/5'-NT/CD73; Recombinant Human 5'-Nucleotidase/5'-NT/CD73 (AAH65937.1; C-Fc); 5'-Nucleotidase; 5'-NT; Ecto-5'-Nucleotidase; CD73; NT5E; NT5; NTE; 5'-NT/CD73 active protein
Host
Human Cells
Purity/Purification
>95% as determined by reducing SDS-PAGE
Form/Format
Supplied as a 0.2um filtered solution of 20mM Tris-HCl, 120mM NaCl, 4mM CaCl2, 20% Glycerol, pH7.5.
Sequence
Trp27-Lys547
Species
Human
Tag
C-Fc
Endotoxin
<1.0EU per ug as determined by the LAL method.
Protein Construction
Recombinant Human 5'-Nucleotidase is produced by our Mammalian expression system and the target gene encoding Trp27-Lys547 is expressed with a Fc tag at the C-terminus.
Activity
Immobilized Human CD73-Fc at 2ug/ml (100ul/well) can bind Anti-Human CD73 mAb* .*: Biotinylated by NHS-biotin prior to testing. The ED50 of Anti-Human CD73 mAb* is 0.31 ug/ml.
Preparation and Storage
Store at < -20 degree C, stable for 6 months. Please minimize freeze-thaw cycles.
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20 degree C.
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20 degree C.
SDS-Page
SDS-Page
Activity
Activity
Related Product Information for 5'-NT/CD73 active protein
CD73 is a glycosyl phosphatidylinositol (GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity. Defects in NT5E are the cause of calcification of joints and arteries (CAJA). The recombinant CD73 lacking GPI anchor is secreted as a monomer.
NCBI and Uniprot Product Information
Molecular Weight
Mol. Mass: 84.6kDa
AP Mol. Mass: 85-100kDa
AP Mol. Mass: 85-100kDa
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Product Notes
The 5'-NT/CD73 (Catalog #AAA2569068) is an Active Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Trp27-Lys5 47. It is sometimes possible for the material contained within the vial of "5'-Nucleotidase/5'-NT/CD73, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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