Typical Testing Data/Standard Curve (for reference only)
Human 5-NT (5-Nucleotidase) CLIA Kit | 5-NT clia kit
Human 5-NT (5-Nucleotidase) CLIA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to 5-NT. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for 5-NT and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain 5-NT, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of 5-NT. You can calculate the concentration of 5-NT in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
may hydrolyze extracellular nucleotides into membrane permeable nucleosides [RGD, Feb 2006]
Uniprot Description
NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.1.3.5; Membrane protein, GPI anchor; Membrane protein, integral; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Nucleotide Metabolism - purine; Phosphatase (non-protein); Nucleotide Metabolism - pyrimidine
Cellular Component: cell surface; membrane; cytoplasm; integral to membrane; plasma membrane
Molecular Function: 5'-nucleotidase activity; ferrous iron binding; nucleotide binding
Biological Process: leukocyte adhesion; negative regulation of inflammatory response; adenosine biosynthetic process; purine nucleotide biosynthetic process; positive regulation of lipid biosynthetic process; brain development; adenosine metabolic process; protein amino acid dephosphorylation; response to aluminum ion; AMP catabolic process
Research Articles on 5-NT
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Product Notes
The Human 5-NT nt5e (Catalog #AAA2531963) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2531963 CLIA Kit recognizes Human 5-NT. It is sometimes possible for the material contained within the vial of "5-NT (5-Nucleotidase), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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