Chicken Yorkie homolog (YAP1) ELISA Kit | YAP1 elisa kit

Chicken Yorkie homolog (YAP1) ELISA Kit

Cat. Num. AAA286629

• Gene Names: YAP1; YAP65
• Reactivity: Chicken
• Synonyms: Yorkie homolog (YAP1); Chicken Yorkie homolog (YAP1) ELISA Kit; YAP; YAP2; YAP65; YKI; yes-associated protein 2; YAP1 elisa kit

• Reactivity: Chicken
• Specificity: This assay has high sensitivity and excellent specificity for detection of Chicken YAP1. No significant cross-reactivity or interference between Chicken YAP1 and analogues was observed.
• Sequence Length: 448

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for YAP1 elisa kit

Description: This assay employs a two-site sandwich ELISA to quantitate YAP1 in samples. An antibody specific for YAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any YAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YAP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: YAP1 contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction.
The human and mouse cDNAs encode deduced 493- and 531-amino acid proteins, respectively, with significant sequence similarity to the chicken protein. All 3 contain a putative protein module of 38 amino acids that is found in various structural, regulatory, and signaling molecules in yeast, nematode, and mammals and may be involved in protein-protein interaction. The mouse protein contains an imperfect repeat of the module. Because one of the prominent features of the sequence motif is 2 tryptophans, it has been designated the WW domain.

NCBI and Uniprot Product Information

• NCBI GI #: 45382559
• NCBI GeneID: 396171
• NCBI Accession #: NP_990574.1
• NCBI GenBank Nucleotide #: NM_205243.1
• UniProt Accession #: P46936
• Molecular Weight: 47,822 Da
• NCBI Official Full Name: transcriptional coactivator YAP1
• NCBI Official Synonym Full Names: Yes associated protein 1
• NCBI Official Symbol: YAP1
• NCBI Official Synonym Symbols: YAP65
• NCBI Protein Information: transcriptional coactivator YAP1
• UniProt Protein Name: Transcriptional coactivator YAP1
• Protein Family: Transcriptional coactivator
• UniProt Gene Name: YAP1
• UniProt Synonym Gene Names: YAP65; YAP65

Uniprot Description

Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (). Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation ().