Immunocytochemistry (ICC)
(SARS-CoV-2 transfected HEK293 cells showing specific staining of SARS-CoV-2 in red. Nuclei are visualized in blue with DAPI.)
Mouse COVID 19 Spike Protein ACE2 Binding Domain Coronavirus Monoclonal Antibody | anti-COVID-19 antibody
SARS-CoV-2 S-Protein ACE2 Binding Domain
Applications
Western Blot, Immunocytochemistry
Purity
Protein G purified culture supernatant
Synonyms
COVID 19 Spike Protein ACE2 Binding Domain Coronavirus; Monoclonal Antibody; SARS-CoV-2 S-Protein ACE2 Binding Domain; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike Protein ACE2 Binding Domain; anti-COVID-19 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
5G8
Purity/Purification
Protein G purified culture supernatant
Form/Format
100ul in PBS + 50% glycerol + 5 mM sodium azide.
Applicable Applications for anti-COVID-19 antibody
Western Blot (WB), Immunocystochemistry (ICC)
Application Notes
WB: 1:2000
ICC: 1:1000
ICC: 1:1000
Species Positively Tested
SARS-CoV-2
Immunogen Species
SARS-CoV-2
Immunogen Information
Recombinant SARS-CoV2 S-Protein ACE2 binding domain expressed in and purified from E Coli
Antigen
The antigen is a recombinant SARS-CoV-2 S-Protein construct containing the ACE2 binding domain expressed in and purified from E Coli. The antibody is Protein G purified culture supernatant.
Preparation and Storage
Storage at -20 degree C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20 degree C.
Shipping: Blue Ice
Country of Origin: USA
Shipping: Blue Ice
Country of Origin: USA
Immunocytochemistry (ICC)
(SARS-CoV-2 transfected HEK293 cells showing specific staining of SARS-CoV-2 in red. Nuclei are visualized in blue with DAPI.)
Immunocytochemistry (ICC)
(SARS-CoV-2 transfected HEK293 cells showing specific staining of SARS-CoV-2 in red. Nuclei are visualized in blue with DAPI.)
Western Blot (WB)
(Western blot showing specific labeling of SARS-CoV-2 at ~ 25k in green in lane 3. Lane 4 shows GFP-tagged SARS-CoV-2 at ~ 50k in green and lane 2 shows no CoV-2 staining in untransfected cells. The red bands show staining with HSP60 as a loading control.)
Western Blot (WB)
(Western blot showing specific labeling of SARS-CoV-2 at ~ 25k in green in lane 3. Lane 4 shows GFP-tagged SARS-CoV-2 at ~ 50k in green and lane 2 shows no CoV-2 staining in untransfected cells. The red bands show staining with HSP60 as a loading control.)
Related Product Information for anti-COVID-19 antibody
The novel SARS-coronavirus 2 (SARS-CoV-2) which causes the disease COVID-19 has been shown to utilize the SARS-CoV receptor ACE2 for entry into human cells (Hoffman, M. et al., Cell 2020). The entry of a coronavirus into host cells is mediated by the viral surface-anchored transmembrane spike (S) glycoprotein which is composed of two functional subunits, S1 which binds the receptor and S2 which fuses the membrane (Walls, AC et.al., Cell 2020). S1 contains a receptor-binding domain (RBD) which specifically recognizes ACE2 as its receptor (Wan, Y. et al., 2020).
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
25/142kDa
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Product Notes
The COVID-19 (Catalog #AAA502237) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's COVID 19 Spike Protein ACE2 Binding Domain Coronavirus can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocystochemistry (ICC). WB: 1:2000 ICC: 1:1000. Researchers should empirically determine the suitability of the COVID-19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COVID 19 Spike Protein ACE2 Binding Domain Coronavirus, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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