Cadherin, Epithelial (CDHE) Recombinant Protein | CDHE recombinant protein

Recombinant Cadherin, Epithelial (CDHE)

Cat. Num. AAA2009265

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Cadherin; Epithelial (CDHE); Recombinant Cadherin; CDHE recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.; MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGSEF-DNAPIFN PSTYQGQVLE NEVGARIATL KVTDDDAPNT PAWNAVYTVV NDPDHQFTVI TDPKTNEGIL KTAKGLDFEA KQQYILHVTV ENEEPFEGSL VPSTATVTVD VVDVNEAPIF VPAEKRVEVP EDFGVGLEIA SYTAREPDTF MEQKITYRIW RDTANWLEIN PETGVISTRA EMDREDSEHV KNSTYTALII ATDDGSPIAT GTGTLLLVLS DVNDNAPIPE PRNMQFCQRN PKPHVITILD PDLPPNTS
• Sequence Length: 886

• Applicable Applications for CDHE recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Asp374~Ser628 (Accession # Q9R0T4) with two N-terminal Tags, His-tag and S-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 13786164
• NCBI GeneID: 83502
• NCBI Accession #: NP_112624.1
• NCBI GenBank Nucleotide #: NM_031334.1
• UniProt Accession #: Q9R0T4; O35794; Q9JIV9
• Molecular Weight: 33.9kDa
• NCBI Official Full Name: cadherin-1
• NCBI Official Synonym Full Names: cadherin 1
• NCBI Official Symbol: Cdh1
• NCBI Protein Information: cadherin-1; E-cadherin; uvomorulin; epithelial cadherin
• UniProt Protein Name: Cadherin-1
• Protein Family: Acetyl-CoA decarbonylase/synthase complex
• UniProt Gene Name: Cdh1
• UniProt Synonym Gene Names: E-cadherin
• UniProt Entry Name: CADH1_RAT

NCBI Description

cell-cell adhesion molecule; may play a role in axonal growth and synase formation [RGD, Feb 2006]

Uniprot Description

CDH1: a single-pass type I membrane protein, and calcium dependent cell adhesion proteins. It is a ligand for integrin alpha-E/beta-7, and it colocalizes with DLG7 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Defects in CDH1 are involved in dysfunction of the cell-cell adhesion system, triggering cancer invasion (gastric, breast, ovary, endometrium and thyroid) and metastasis. Has a potent invasive suppressor role.

Protein type: Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis; Ubiquitin ligase

Cellular Component: apical junction complex; internal side of plasma membrane; cell surface; focal adhesion; basolateral plasma membrane; lateral loop; integral to membrane; intercellular junction; trans-Golgi network; catenin complex; actin cytoskeleton; cell-cell adherens junction; adherens junction; axon; apical part of cell; perinuclear region of cytoplasm; cytoplasm; plasma membrane; nerve terminal; cell junction; lateral plasma membrane; endosome

Molecular Function: protein domain specific binding; protein binding; gamma-catenin binding; beta-catenin binding; GTPase activating protein binding; cell adhesion molecule binding; ankyrin binding; calcium ion binding; glycoprotein binding; protein phosphatase binding

Biological Process: response to drug; in utero embryonic development; positive regulation of transcription, DNA-dependent; response to toxin; regulation of caspase activity; trophectodermal cell differentiation; response to organic substance; regulation of water loss via skin; cell-cell adhesion; synaptogenesis; regulation of protein localization; sensory perception of sound; pituitary gland development; calcium-dependent cell-cell adhesion; protein metabolic process; positive regulation of transcription factor import into nucleus; negative regulation of cell-cell adhesion; homophilic cell adhesion; protein homooligomerization; neurite development; negative regulation of epithelial cell proliferation