CD6 recombinant protein

CD6 Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8118691

• Gene Names: CD6; TP120
• Purity: >95 % as determined by SDS-PAGE.
• Synonyms: CD6; CD6 Protein; Human; Recombinant (hFc Tag); Human CD6/Cluster of Differentiation 6 Protein (Fc Tag); CD6 molecule; TP120 Protein; CD6 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95 % as determined by SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 619aa
• Sequence: Met1-Glu398

• Species: Human
• Tag: hFc
• Endotoxin: <1.0EU per ug protein as determined by the LAL method
• Predicted N Terminal: His18
• Protein Construction: |A DNA sequence encoding the human CD6 (NP_006716.3) (Met1-Glu398) was expressed with the Fc region of human IgG1 at the C-terminus
• Activity: Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells.When 8x10E4 cells/well are added to CD6-Fc coated plates (10ug/mL, 100uL/well), approximately >70% will adhere after 60 minutes at 37 degree C.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Testing Data

Related Product Information for CD6 recombinant protein

T-cell differentiation antigen CD6, also known as TP12 and CD6, is a single-pass type I membrane protein which contains threeSRCR domains. CD6/TP12 is a cell surface glycoprotein expressed primarily on T cells, it may function as a costimulatory molecule and may play a role in autoreactive immune responses. CD6/TP12 is expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain. CD6 ligand termed CD166 (previously known as activated leukocyte cell adhesion molecule, ALCAM) has been identified and shown to be expressed on activated T cells, B cells, thymic epithelium, keratinocytes, and in rheumatoid arthritis synovial tissue. CD6/TP12 binds to activated leukocyte cell adhesion molecule (CD166), and is considered as a costimulatory molecule involved in lymphocyte activation and thymocyte development. CD6/TP12 partially associates with the TCR/CD3 complex and colocalizes with it at the center of the mature immunological synapse (IS) on T lymphocytes. During thymic development CD6-dependent signals may contribute both to thymocyte survival, and to the overall functional avidity of selection in both man and mouse.

Product Categories/Family for CD6 recombinant protein

ITIM/ITAM Immunoreceptors and Related Molecules

References

Joo YS. et al., 2000, Arthritis Rheum. 43 (2): 329-35. Singer NG. et al., 2002, Int Immunol. 14 (6): 585-97. Gimferrer I. et al., 2005, J Immunol. 175 (3): 1406-14. Alonso R. et al., 2010, J Autoimmun. 35 (4): 336-41.

NCBI and Uniprot Product Information

• NCBI GI #: 194018748
• NCBI GeneID: 923
• NCBI Accession #: NP_006716.3
• NCBI GenBank Nucleotide #: NM_006725.4
• UniProt Accession #: P30203; Q9UMF2; Q9Y4K7; Q9Y4K8; Q9Y4K9; Q9Y4L0; A4KAD4; A4KAD5
• Molecular Weight: 60,572 Da
• NCBI Official Full Name: T-cell differentiation antigen CD6 isoform 1
• NCBI Official Synonym Full Names: CD6 molecule
• NCBI Official Symbol: CD6
• NCBI Official Synonym Symbols: TP120
• NCBI Protein Information: T-cell differentiation antigen CD6; T12; CD6 antigen
• UniProt Protein Name: T-cell differentiation antigen CD6
• Protein Family: CD63 antigen
• UniProt Gene Name: CD6
• UniProt Entry Name: CD6_HUMAN

NCBI Description

This gene encodes a protein found on the outer membrane of T-lymphocytes as well as some other immune cells. The encoded protein contains three scavenger receptor cysteine-rich (SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule. The gene product is important for continuation of T cell activation. This gene may be associated with susceptibility to multiple sclerosis (PMID: 19525953, 21849685). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

CD6: a type I membrane protein involved in cell adhesion. Binds to CD166. Expressed on thymocytes, peripheral T cells, a subset of B cells, and a subset of neurons. Mediates the binding of developing thymocytes with thymic epithelial cells. CD6- T cells are less autoreactive than CD6+ T cells. Phosphorylated on tyrosines and serines following T cell antigen receptor signaling. Five splice-variant isoforms have been described.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: integral to plasma membrane

Molecular Function: protein binding; scavenger receptor activity

Biological Process: receptor-mediated endocytosis; cell adhesion