Western Blot (WB)
(Western Blot analysis in HepG2, CHO, K562, HeLa cell line and mouse liver tissue lysates (35ug/lane) using MBS6006433. This demonstrates that MBS6006433 detected the HSP90B1 protein (arrow).)
Rabbit HSP90B1 Polyclonal Antibody | anti-HSP90B1 antibody
HSP90B1, NT (HSP90B1, GRP94, TRA1, Endoplasmin, 94kD glucose-regulated protein, Heat shock protein 90kD beta member 1, Tumor rejection antigen 1, gp96 homolog)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Immunofluorescence: 1:10-50
Western Blot (WB)
(Western Blot analysis in HepG2, CHO, K562, HeLa cell line and mouse liver tissue lysates (35ug/lane) using MBS6006433. This demonstrates that MBS6006433 detected the HSP90B1 protein (arrow).)
Western Blot (WB)
(Western Blot analysis in HepG2, CHO, K562, HeLa cell line and mouse liver tissue lysates (35ug/lane) using MBS6006433. This demonstrates that MBS6006433 detected the HSP90B1 protein (arrow).)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human lung using MBS6006433 carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6006433 for immunohistochemistry.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human lung using MBS6006433 carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6006433 for immunohistochemistry.)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of K562 cells using MBS6006433 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of K562 cells using MBS6006433 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis with NCI-H460 cells using MBS6006433 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis with NCI-H460 cells using MBS6006433 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. [provided by RefSeq, Aug 2012]
Uniprot Description
GRP94: Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. Homodimer; disulfide-linked. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with AIMP1; regulates its retention in the endoplasmic reticulum. Interacts with OS9. Interacts with CNPY3. This interaction is disrupted in the presence of ATP. Interacts with TLR4 and TLR9, but not with TLR3. Belongs to the heat shock protein 90 family.
Protein type: Apoptosis; Heat shock protein; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 12q24.2-q24.3
Cellular Component: endoplasmic reticulum membrane; focal adhesion; endoplasmic reticulum; endoplasmic reticulum lumen; extracellular region; cytosol; extracellular matrix; membrane; perinuclear region of cytoplasm; plasma membrane; melanosome; midbody; nucleus
Molecular Function: protein binding; low-density lipoprotein receptor binding; RNA binding; unfolded protein binding; virion binding; calcium ion binding; protein phosphatase binding; ATP binding
Biological Process: actin rod formation; receptor-mediated endocytosis; ER-associated protein catabolic process; protein transport; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; unfolded protein response; sequestering of calcium ion; response to hypoxia; toll-like receptor signaling pathway; innate immune response; regulation of phosphoprotein phosphatase activity; negative regulation of apoptosis
Research Articles on HSP90B1
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Product Notes
The HSP90B1 hsp90b1 (Catalog #AAA6006433) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HSP90B1, NT (HSP90B1, GRP94, TRA1, Endoplasmin, 94kD glucose-regulated protein, Heat shock protein 90kD beta member 1, Tumor rejection antigen 1, gp96 homolog) reacts with Hamster, Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HSP90B1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF). Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Flow Cytometry: 1:10-50 Immunofluorescence: 1:10-50. Researchers should empirically determine the suitability of the HSP90B1 hsp90b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSP90B1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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