Rabbit anti-Human, mouse Diacylglycerol kinase iota (DGKI) Polyclonal Antibody | anti-DGKI antibody

Diacylglycerol kinase iota (DGKI) Antibody (C-term)

Cat. Num. AAA9209231

• Gene Names: DGKI; DGK-IOTA
• Reactivity: Human, mouse
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Diacylglycerol kinase iota (DGKI); Polyclonal Antibody; Diacylglycerol kinase iota (DGKI) Antibody (C-term); Diacylglycerol kinase iota; DAG kinase iota; Diglyceride kinase iota; DGK-iota; DGKI; anti-DGKI antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This Diacylglycerol kinase iota (DGKI) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 890-920 amino acids from the C-terminal region of human Diacylglycerol kinase iota (DGKI).
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

• Applicable Applications for anti-DGKI antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: WB~~1:1000; IHC-P~~1:50~100
• Cellular Location: Cytoplasm. Nucleus.
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(The anti-DGKI Pab is used in Western blot to detect DGKI in mouse cerebellum tissue lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(DGKI Antibody (Y905) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the DGKI antibody detected the DGKI protein (arrow).)

Related Product Information for anti-DGKI antibody

DGKI a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases regulate the intracellular concentration of diacylglycerol through its phosphorylation, producing phosphatidic acid. The specific role of the enzyme encoded by this gene is undetermined, however, it may play a crucial role in the production of phosphatidic acid in the retina or in recessive forms of retinal degeneration.

Product Categories/Family for anti-DGKI antibody

Signal Transduction

References

Ding, L., et al., J. Biol. Chem. 273(49):32746-32752 (1998).
Bowne, S.J., et al., Mol. Vis. 6, 6-9 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 4758156
• NCBI GeneID: 9162
• NCBI Accession #: NP_004708.1
• NCBI GenBank Nucleotide #: NM_004717.2
• UniProt Accession #: O75912; Q9NZ49; A4D1Q9
• Molecular Weight: 116997
• NCBI Official Full Name: diacylglycerol kinase iota
• NCBI Official Synonym Full Names: diacylglycerol kinase, iota
• NCBI Official Symbol: DGKI
• NCBI Official Synonym Symbols: DGK-IOTA
• NCBI Protein Information: diacylglycerol kinase iota
• UniProt Protein Name: Diacylglycerol kinase iota
• Protein Family: Diacylglycerol kinase
• UniProt Gene Name: DGKI
• UniProt Synonym Gene Names: DAG kinase iota; DGK-iota
• UniProt Entry Name: DGKI_HUMAN

NCBI Description

This gene is a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases regulate the intracellular concentration of diacylglycerol through its phosphorylation, producing phosphatidic acid. The specific role of the enzyme encoded by this gene is undetermined, however, it may play a crucial role in the production of phosphatidic acid in the retina or in recessive forms of retinal degeneration. [provided by RefSeq, Jul 2008]

Uniprot Description

DGKI: a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases regulate the intracellular concentration of diacylglycerol through its phosphorylation, producing phosphatidic acid. The specific role of the enzyme encoded by undetermined, however, it may play a crucial role in the production of phosphatidic acid in the retina or in recessive forms of retinal degeneration. [provided by RefSeq, Jul 2008]

Protein type: Motility/polarity/chemotaxis; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - glycerolipid; EC 2.7.1.107; Kinase, lipid

Chromosomal Location of Human Ortholog: 7q32.3-q33

Cellular Component: nucleoplasm; perinuclear region of cytoplasm; cytoplasm; plasma membrane; dendritic spine; synapse; nucleus

Molecular Function: protein binding; Ras GTPase binding; metal ion binding; diacylglycerol kinase activity; ATP binding; GTPase inhibitor activity; NAD+ kinase activity

Biological Process: platelet activation; negative regulation of catalytic activity; protein kinase C activation; positive regulation of Ras protein signal transduction; blood coagulation; phosphorylation