Rabbit anti-Human, Mouse MRGPRX2 Polyclonal Antibody | anti-MRGPRX2 antibody

MRGPRX2 Antibody

Cat. Num. AAA7006480

• Gene Names: MRGPRX2; MGRG3; MRGX2
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: MRGPRX2; Polyclonal Antibody; MRGPRX2 Antibody; Mas-related G-protein coupled receptor member X2; MRGX2; anti-MRGPRX2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid

• Applicable Applications for anti-MRGPRX2 antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:1000-1:5000; IF: 1:50-1:200
• Immunogen: Recombinant Human Mas-related G-protein coupled receptor member X2 protein (286-330AA)
• Conjugate: Non-conjugated
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Immunogen Species: Homo sapiens (Human)
• Research Area: Neuroscience
• Target Names: MRGPRX2
• Preparation and Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western Blot (WB)

(All lanes: MRGPRX2 antibody at 5ug/mlLane 1: 293T whole cell lysateLane 2: Mouse liver tissueSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 38 kDaObserved band size: 38 kDa)

Immunofluorescence (IF)

(Immunofluorescent analysis of MCF-7 cells using MBS7006480 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Western Blot (WB)

(Western BlotPositive WB detected in: MCF-7 whole cell lysate, 293 whole cell lysate, HL60 whole cell lysate, K562 whole cell lysateAll lanes: MRGPRX2 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 38 kDaObserved band size: 38 kDa)

NCBI and Uniprot Product Information

• NCBI GI #: 746816153
• NCBI GeneID: 117194
• NCBI Accession #: NP_001290544.1
• NCBI GenBank Nucleotide #: NM_001303615.1
• UniProt Accession #: Q96LB1; Q4QXW4; Q4QXW7; Q4QXX0; Q4QXX2; Q4QXX3; Q4QXX4; Q4QXX6; Q4QXX7; B5B0C7; W8W3L5
• NCBI Official Full Name: mas-related G-protein coupled receptor member X2
• NCBI Official Synonym Full Names: MAS related GPR family member X2
• NCBI Official Symbol: MRGPRX2
• NCBI Official Synonym Symbols: MGRG3; MRGX2
• NCBI Protein Information: mas-related G-protein coupled receptor member X2
• UniProt Protein Name: Mas-related G-protein coupled receptor member X2
• Protein Family: Mas-related G-protein coupled receptor
• UniProt Gene Name: MRGPRX2
• UniProt Synonym Gene Names: MRGX2
• UniProt Entry Name: MRGX2_HUMAN

Uniprot Description

MRGPRX2: Orphan receptor. Probably involved in the function of nociceptive neurons. May regulate nociceptor function and/or development, including the sensation or modulation of pain. Cortistatin-14 seems to be a high potency ligand at this receptor. Cortistatin has several biological functions including roles in sleep regulation locomotor activity, and cortical function. In receptor-expressing cells, cortistatin-stimulated increases in intracellular Ca(2+) but had no effect on basal or forskolin- stimulated cAMP levels, suggesting that this receptor is G(q)- coupled. Belongs to the G-protein coupled receptor 1 family. Mas subfamily.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR; GPCR, family 1

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: integral to membrane; integral to plasma membrane; mast cell granule

Molecular Function: G-protein coupled receptor activity; neuropeptide binding

Biological Process: G-protein coupled receptor protein signaling pathway; mast cell activation; mast cell degranulation; positive regulation of cytokinesis; sensory perception of pain; sleep