CD6 recombinant protein

Recombinant human CD6 protein

Cat. Num. AAA201318

• Gene Names: CD6; TP120
• Applications: SDS-Page
• Purity: 90% by SDS-PAGE
• Synonyms: CD6; Recombinant human CD6 protein; T-cell differentiation antigen CD6; T12; TP120; CD_antigen; T-cell differentiation antigen CD6 isform1; CD6 recombinant protein

• Host: Baculovirus
• Purity/Purification: 90% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 18-402aa
• Sequence: HPSPAPP DQLNTSSAES ELWEPGERLP VRLTNGSSSC SGTVEVRLEA SWEPACGALW DSRAAEAVCR ALGCGGAEAA SQLAPPTPEL PPPPAAGNTS VAANATLAGA PALLCSGAEW RLCEVVEHAC RSDGRRARVT CAENRALRLV DGGGACAGRV EMLEHGEWGS VCDDTWDLED AHVVCRQLGC GWAVQALPGL HFTPGRGPIH RDQVNCSGAE AYLWDCPGLP GQHYCGHKED AGAVCSEHQS WRLTGGADRC EGQVEVHFRG VWNTVCDSEW YPSEAKVLCQ SLGCGTAVER PKGLPHSLSG RMYYSCNGEE LTLSNCSWRF NNSNLCSQSL AARVLCSASR SLHNLSTPEV PASVQTVTIE SSVTVKIENK ESRELMLL

• Applicable Applications for CD6 recombinant protein: SDS-PAGE, Bioactivity
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Human
• Tag: hIgG-His-Tag
• Predicted N-term: Ala
• Bioactivity: Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When cells are added to human CD6 coated plates 10ug/ml. This effect is more to 50%.
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for CD6 recombinant protein

CD6, also known as T-cell differentiation antigen CD6, is a member of the group B SRCR (scavenger receptor cysteine-rich) superfamily. CD6 is a cell surface glycoprotein expressed primarily on T cells and It expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain. And T-cell differentiation antigen CD6 appears to play a role as both a co-stimulatory molecule in T cell activation and as an adhesion receptor. CD6 binds to CD166(known as ALCAM), and is considered as a costimulatory molecule involved in lymphocyte activation and thymocyte development.. Certain alleles of this protein may be associated with susceptibility to  multiple sclerosis. Recombinant human CD6, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for CD6 recombinant protein

Immunology; Proteins

References

Singer, N.G. et al. (1996) Immunology 88:537-547. Namir J Hassan et al. (2004) Eur J Immunol. 34:930-940.

NCBI and Uniprot Product Information

• NCBI GI #: 194018748
• NCBI GeneID: 923
• NCBI Accession #: NP_006716
• NCBI GenBank Nucleotide #: NM_006725
• UniProt Accession #: P30203
• Molecular Weight: 71,889 Da
• NCBI Official Full Name: T-cell differentiation antigen CD6 isoform 1
• NCBI Official Synonym Full Names: CD6 molecule
• NCBI Official Symbol: CD6
• NCBI Official Synonym Symbols: TP120
• NCBI Protein Information: T-cell differentiation antigen CD6; CD6 antigen; T12
• UniProt Protein Name: T-cell differentiation antigen CD6
• Protein Family: CD63 antigen
• UniProt Gene Name: CD6
• UniProt Entry Name: CD6_HUMAN

Uniprot Description

CD6: a type I membrane protein involved in cell adhesion. Binds to CD166. Expressed on thymocytes, peripheral T cells, a subset of B cells, and a subset of neurons. Mediates the binding of developing thymocytes with thymic epithelial cells. CD6- T cells are less autoreactive than CD6+ T cells. Phosphorylated on tyrosines and serines following T cell antigen receptor signaling. Five splice-variant isoforms have been described.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: integral to plasma membrane

Molecular Function: protein binding; scavenger receptor activity

Biological Process: receptor-mediated endocytosis; cell adhesion