Western Blot (WB)
(Western blot analysis of lysate from Hela cell line, using FUT8 Antibody (Center). MBS9211105 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
Rabbit FUT8 Polyclonal Antibody | anti-FUT8 antibody
FUT8 Antibody (Center)
Western Blot (WB)
(Western blot analysis of lysate from Hela cell line, using FUT8 Antibody (Center). MBS9211105 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
Western Blot (WB)
(Western blot analysis of lysate from Hela cell line, using FUT8 Antibody (Center). MBS9211105 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
Western Blot (WB)
(FUT8 Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the FUT8 antibody detected the FUT8 protein (arrow).)
Western Blot (WB)
(FUT8 Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the FUT8 antibody detected the FUT8 protein (arrow).)
Immunohistochemistry (IHC)
(FUT8 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FUT8 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(FUT8 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FUT8 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
The product of this gene catalyzes the transfer of fucose from
GDP-fucose to N-linked type complex glycopeptides. This enzyme is
distinct from other fucosyltransferases which catalyze alpha1-2,
alpha1-3, and alpha1-4 fucose addition. The expression of this gene
may contribute to the malignancy of cancer cells and to their
invasive and metastatic capabilities. Alternatively spliced
variants encoding different isoforms have been identified.
Wang, X., et al. J. Biochem. 145(5):643-651(2009)
Kudo, T., et al. Mol. Cancer 6, 32 (2007) :
Ihara, H., et al. Glycobiology 16(4):333-342(2006)
Ito, Y., et al. Cancer Lett. 200(2):167-172(2003)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an enzyme belonging to the family of fucosyltransferases. The product of this gene catalyzes the transfer of fucose from GDP-fucose to N-linked type complex glycopeptides. This enzyme is distinct from other fucosyltransferases which catalyze alpha1-2, alpha1-3, and alpha1-4 fucose addition. The expression of this gene may contribute to the malignancy of cancer cells and to their invasive and metastatic capabilities. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2011]
Uniprot Description
FUT8: Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans. Belongs to the glycosyltransferase 23 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Glycan Metabolism - N-glycan biosynthesis; EC 2.4.1.68; Transferase; Glycan Metabolism - keratan sulfate biosynthesis; Motility/polarity/chemotaxis; Membrane protein, integral
Chromosomal Location of Human Ortholog: 14q24.3
Cellular Component: Golgi membrane; Golgi apparatus; membrane; cytoplasm; integral to membrane
Molecular Function: glycoprotein 6-alpha-L-fucosyltransferase activity; SH3 domain binding
Biological Process: integrin-mediated signaling pathway; cell migration; in utero embryonic development; receptor metabolic process; oligosaccharide biosynthetic process; protein amino acid N-linked glycosylation; respiratory gaseous exchange; post-translational protein modification; GDP-L-fucose metabolic process; cellular protein metabolic process; transforming growth factor beta receptor signaling pathway; protein amino acid N-linked glycosylation via asparagine; protein amino acid glycosylation in Golgi; N-glycan processing; L-fucose catabolic process
Research Articles on FUT8
Similar Products
Product Notes
The FUT8 fut8 (Catalog #AAA9211105) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 329-357. The FUT8 Antibody (Center) reacts with Human (Predicted Reactivity: Bovine, Mouse, Pig, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's FUT8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the FUT8 fut8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FUT8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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