Mouse CD6 Monoclonal Antibody | anti-CD6 antibody

MOUSE ANTI HUMAN CD6:FITC

Cat. Num. AAA210795

• Gene Names: CD6; TP120
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD6; Monoclonal Antibody; MOUSE ANTI HUMAN CD6:FITC; anti-CD6 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: MEM-98
• Form/Format: FITC; Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
• Concentration: IgG concentration 0.1mg/ml (varies by lot)
• Sequence Length: 601

• Applicable Applications for anti-CD6 antibody: Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood ; Flow Cytometry: Maximum Dilution: Neat
• Perservative Stabilisers: 0.09% Sodium Azide; 1% Bovine Serum Albumin; Preparation
• Buffer Solution: Phosphate buffered saline
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD6 (MBS212366))

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD6:FITC)

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD6:RPE (MBS213686))

Related Product Information for anti-CD6 antibody

Mouse anti Human CD6 antibody, clone MEM-98 recognizes human CD6, also known as T12 or TP120. CD6 is a ~100-130kDa type 1 single pass trans-membrane protein member of the immunoglobulin superfamily. CD6 possesses 3 scavenger receptor cysteine rich (SRCR) domains in it's extracellular sequence. The membrane proximal SRCR3 contains the epitope responsible for interaction with CD166, also known as Activated Leukocyte Cell Adhesion Molecule (ALCAM) or CD166. Multiple gene transcripts have been detected encoding CD6 in man resulting in the production of a number of CD6 isoforms. Mouse anti human CD6, clone MEM-98 recognizes an external epitope located in the membrane-distal SRCR domain 1 and is expected to recognize all CD6 isoforms so far identified, CD6A-E. CD6 has been implicated as a therapeutic target for a number of autoimmune conditions (Pinto et al. 2013) including Sj¶gren's syndrome (Ramos-Casals et al. 2001), rheumatoid arthritis (Rodriguez et al.) and psoriasis (Wilsmann-Theis et al. 2006).

NCBI and Uniprot Product Information

• NCBI GI #: 363543322
• NCBI GeneID: 923
• NCBI Accession #: NP_001241679.1
• NCBI GenBank Nucleotide #: NM_001254750.1
• UniProt Accession #: P30203; Q9UMF2; Q9Y4K7; Q9Y4K8; Q9Y4K9; Q9Y4L0; A4KAD4; A4KAD5
• Molecular Weight: 60,572 Da
• NCBI Official Full Name: T-cell differentiation antigen CD6 isoform 2
• NCBI Official Synonym Full Names: CD6 molecule
• NCBI Official Symbol: CD6
• NCBI Official Synonym Symbols: TP120
• NCBI Protein Information: T-cell differentiation antigen CD6; CD6 antigen; T12
• UniProt Protein Name: T-cell differentiation antigen CD6
• Protein Family: CD63 antigen
• UniProt Gene Name: CD6
• UniProt Entry Name: CD6_HUMAN

NCBI Description

This gene encodes a protein found on the outer membrane of T-lymphocytes as well as some other immune cells. The encoded protein contains three scavenger receptor cysteine-rich (SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule. The gene product is important for continuation of T cell activation. This gene may be associated with susceptibility to multiple sclerosis (PMID: 19525953, 21849685). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

CD6: a type I membrane protein involved in cell adhesion. Binds to CD166. Expressed on thymocytes, peripheral T cells, a subset of B cells, and a subset of neurons. Mediates the binding of developing thymocytes with thymic epithelial cells. CD6- T cells are less autoreactive than CD6+ T cells. Phosphorylated on tyrosines and serines following T cell antigen receptor signaling. Five splice-variant isoforms have been described.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: integral to plasma membrane

Molecular Function: protein binding; scavenger receptor activity

Biological Process: receptor-mediated endocytosis; cell adhesion