Flow Cytometry (FC/FACS)
CD152 (CTLA-4) Recombinant Protein | CD152 recombinant protein
CD152 (CTLA-4)-muIg
Applications
Flow Cytometry, Functional Assay, ELISA
Purity
Human CD152-muIg fusion protein from tissue culture supernatant of BHK transfectants was Protein A purified to >95% by SDS-PAGE (<1% bovine immunoglobulin), and conjugated to Allophycocyanin through a sulfo-ester linkage. Unconjugated fusion protein was r
Synonyms
CD152 (CTLA-4); CD152 (CTLA-4)-muIg; CD152(CTLA-4)-muIgAllophycocyanin; Human CD152(CTLA-4)-muIg/APC Fusion Protein*; CD152 recombinant protein
Purity/Purification
Human CD152-muIg fusion protein from tissue culture supernatant of BHK transfectants was Protein A purified to >95% by SDS-PAGE (<1% bovine immunoglobulin), and conjugated to Allophycocyanin through a sulfo-ester linkage. Unconjugated fusion protein was r
Form/Format
Allophycocyanin
Applicable Applications for CD152 recombinant protein
Flow Cytometry (FC/FACS), ELISA (EIA)
Buffer
50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative)
Performance
Five x 105 cultured Raji human tumor cells were washed and incubated 45 minutes on ice with 80 ul of CD152-muIg/APC at a dilution factor of 1:20 (10 ug/ml). Cells were washed three times, fixed and analyzed using by FACS. The cells stained positive with a mean shift of 2.2 log10 fluorescent units when compared to background. Binding was blocked when cells were pre incubated with 20 ul of 0.5 mg/ml unconjugated CD152-muIg.
Preparation and Storage
Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Protect from light.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Flow Cytometry (FC/FACS)
Flow Cytometry (FC/FACS)
Related Product Information for CD152 recombinant protein
Human CD152 (CTLA-4) is a cell surface glycoprotein expressed at low levels on activated T cells (1). CD152 is a high affinity receptor for the costimulatory molecules CD80 (B7-1) and CD86 (B7-2) and appears to function as a negative regulator of T cell activation (2, 3). A soluble fusion protein combining the extracellular (125aa) domain of human CD152 and murine IgG2a Fc (CTLA-4 Ig) was developed (4).
Product Categories/Family for CD152 recombinant protein
References
1. T. Lindsten, et al, (1993) J Immunol 151: 3489-3499. 2. T.L. Walunas, et al, (1994) Immunity 1: 405-413. 3. N.J. Karandikar, et al, (1996) J Exp Med 184: 783-788. 4. A.H. Cross, et al, (1995) J Clin Invest 95: 2783-2789. 5. P.A. Morton, et al, (1996) J Immunol 156: 1047-1054. 6). Takamatsu H, RME Parkhouse, et al. (1999) Immunology 97(2):211-213. 7). Le Leduec JB, B Dubois, et al. (2016) Vaccine 34(7): 914-22. PMID: 26768129
Similar Products
Product Notes
The CD152 (Catalog #AAA666917) is a Recombinant Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD152 (CTLA-4) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA). Researchers should empirically determine the suitability of the CD152 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD152 (CTLA-4), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
